Understanding the toxicity mechanism of CuO nanoparticles: the intracellular view of exposed earthworm cells

0545801 - MBÚ 2022 RIV GB eng J - Journal Article
Navarro Pacheco, Natividad Isabel - Roubalová, Radka - Dvořák, Jiří - Benada, Oldřich - Pinkas, Dominik - Kofroňová, Olga - Semerád, Jaroslav - Pivokonský, Martin - Cajthaml, Tomáš - Bilej, Martin - Procházková, Petra
Understanding the toxicity mechanism of CuO nanoparticles: the intracellular view of exposed earthworm cells.
Environmental Science-Nano. Roč. 8, č. 9 (2021), s. 2464-2477. ISSN 2051-8153. E-ISSN 2051-8161
R&D Projects: GA MZe(CZ) QK1910095; GA MŠMT(CZ) LM2018129; GA MŠMT(CZ) EF16_013/0001775
EU Projects: European Commission(XE) 671881 - PANDORA
Institutional support: RVO:61388971 ; RVO:68378050 ; RVO:67985874
Keywords : in-vitro * eisenia-hortensis * comet assay * soil * celomocytes * oxide * bioaccumulation * bioavailability * terrestrial * activation
OECD category: Immunology; Immunology (UMG-J); Environmental sciences (social aspects to be 5.7) (UH-J)
Impact factor: 9.473, year: 2021
Method of publishing: Limited access
https://pubs.rsc.org/en/content/articlelanding/2021/EN/D1EN00080B

Copper oxide nanoparticles (CuO NPs) are widely used in industry. Once released, they can enter the soil system and endanger organisms living in this environment. Therefore, monitoring the NP impact on soil organisms and identification of suitable biomarkers associated with NP pollution are required. In this study, immune effector cells of the earthworm Eisenia andrei, amoebocytes, were exposed to environmentally relevant sublethal concentrations of CuO NPs (1, 10, and 100 mu g mL(-1) of Cu) and their impact on the cellular and subcellular levels, as well as on the mRNA levels of molecules involved in the defense reactions, was assessed in vitro. CuO NPs decreased the viability of both amoebocyte subpopulations by 40% at the highest concentration tested (100 mu g mL(-1) of Cu). Further, CuO NPs caused significant attenuation of the phagocytic function of hyaline amoebocytes after 6 and 24 hours of exposure, by 37 and 25%, respectively. The concentration of the lipid peroxidation subproduct, malondialdehyde, was 10 times elevated in cells exposed to CuO NPs (100 mu g mL(-1) of Cu) after 6 hours of exposure. We hypothesize that malondialdehyde may induce DNA breaks, cell cycle arrest, and subsequent cell death. Electron microscopy showed the interaction between CuO NPs and immune effector cells, amoebocytes. Moreover, aggregates of CuO NPs were shown to be engulfed and located in the cytoplasm of these cells. However, data from all experiments indicate that the observed effects of CuO NPs on earthworm coelomocytes were caused mainly by the dissolved Cu2+ ions derived from nanoparticles (NPs). The determination of effective parameters such as oxidative stress, immune reactivity, and genotoxicity would provide valuable comprehension and data for environmental assessment of NP impact on soil organisms.
Permanent Link: http://hdl.handle.net/11104/0322452