Bioluminescence determination of caspase-3/7 in single osteosarcoma cells

0545800 - ÚIACH 2022 RIV CZ eng C - Conference Paper (international conference)
Killinger, Michael - Matalová, Eva - Klepárník, Karel
Bioluminescence determination of caspase-3/7 in single osteosarcoma cells.
Proceedings of the 17th International Students Conference “Modern Analytical Chemistry”. Praha: Přírodovědecká fakulta Univerzity Karlovy, 2021 - (Nesměrák, K.), s. 56-61. ISBN 978-80-7444-089-2.
[International Students Conference 'Modern Analytical Chemistry' /17./. Praha (CZ), 16.09.2021-17.09.2021]
R&D Projects: GA ČR(CZ) GA20-00726S
Institutional support: RVO:68081715 ; RVO:67985904
Keywords : single-cell analysis * osteoblast * osteosarcoma
OECD category: Analytical chemistry; Developmental biology (UZFG-Y)

The protein heterogeneity at the single-cell level has been recognized to be vital for an understanding of various life processes during animal development. In addition, the knowledge of accurate quantity of relevant proteins at cellular level is essential for appropriate interpretation of diagnostic and therapeutic results. Here, we determined the level of active caspase-3/7 in physiological and pathological (cancer) conditions by our miniaturized instrumentation.
Permanent Link: http://hdl.handle.net/11104/0322451