Mitochondrial Retrograde Signaling Contributes to Metabolic Differentiation in Yeast Colonies

0545739 - MBÚ 2022 RIV CH eng J - Journal Article
Plocek, V. - Fadrhonc, K. - Maršíková, J. - Váchová, Libuše - Pokorná, Alexandra - Hlaváček, Otakar - Wilkinson, D. - Palková, Z.
Mitochondrial Retrograde Signaling Contributes to Metabolic Differentiation in Yeast Colonies.
International Journal of Molecular Sciences. Roč. 22, č. 11 (2021), č. článku 5597. E-ISSN 1422-0067
R&D Projects: GA ČR(CZ) GA19-09381S
Institutional support: RVO:61388971
Keywords : mitochondrial retrograde signaling * yeast colonies * colony development and differentiation * Saccharomyces cerevisiae * proteomic analysis
OECD category: Microbiology
Impact factor: 6.208, year: 2021
Method of publishing: Open access
https://www.mdpi.com/1422-0067/22/11/5597/htm

During development of yeast colonies, various cell subpopulations form, which differ in their properties and specifically localize within the structure. Three branches of mitochondrial retrograde (RTG) signaling play a role in colony development and differentiation, each of them activating the production of specific markers in different cell types. Here, aiming to identify proteins and processes controlled by the RTG pathway, we analyzed proteomes of individual cell subpopulations from colonies of strains, mutated in genes of the RTG pathway. Resulting data, along with microscopic analyses revealed that the RTG pathway predominantly regulates processes in U cells, long-lived cells with unique properties, which are localized in upper colony regions. Rtg proteins therein activate processes leading to amino acid biosynthesis, including transport of metabolic intermediates between compartments, but also repress expression of mitochondrial ribosome components, thus possibly contributing to reduced mitochondrial translation in U cells. The results reveal the RTG pathway's role in activating metabolic processes, important in U cell adaptation to altered nutritional conditions. They also point to the important role of Rtg regulators in repressing mitochondrial activity in U cells.
Permanent Link: http://hdl.handle.net/11104/0322401