Molecular architecture of the endocytic TPLATE complex

0545616 - ÚEB 2022 RIV US eng J - Journal Article
Yperman, K. - Wang, J. - Eeckhout, D. - Winkler, J. - Vu, L. D. - Vandorpe, M. - Grones, P. - Mylle, E. - Kraus, M. - Merceron, R. - Nolf, J. - Mor, E. - de Bruyne, B. - Loris, Remy - Potocký, Martin - Savvides, S. N. - De Rybel, B. - De Jaeger, G. - Van Damme, D. - Pleskot, Roman
Molecular architecture of the endocytic TPLATE complex.
Science Advances. Roč. 7, č. 9 (2021), č. článku eabe7999. ISSN 2375-2548. E-ISSN 2375-2548
R&D Projects: GA ČR(CZ) GA19-21758S
Institutional support: RVO:61389030
Keywords : Cell membranes * Molecular architecture * Structural elucidation
OECD category: Cell biology
Impact factor: 14.980, year: 2021
Method of publishing: Open access
http://doi.org/10.1126/sciadv.abe7999

Eukaryotic cells rely on endocytosis to regulate their plasma membrane proteome and lipidome. Most eukaryotic groups, except fungi and animals, have retained the evolutionary ancient TSET complex as an endocytic regulator. Unlike other coatomer complexes, structural insight into TSET is lacking. Here, we reveal the molecular architecture of plant TSET [TPLATE complex (TPC)] using an integrative structural approach. We identify crucial roles for specific TSET subunits in complex assembly and membrane interaction. Our data therefore generate fresh insight into the differences between the hexameric TSET in Dictyostelium and the octameric TPC in plants. Structural elucidation of this ancient adaptor complex represents the missing piece in the coatomer puzzle and vastly advances our functional as well as evolutionary insight into the process of endocytosis.
Permanent Link: http://hdl.handle.net/11104/0322295