Ph2 encodes the mismatch repair protein MSH7-3D that inhibits wheat homoeologous recombination

0545615 - ÚEB 2022 RIV GB eng J - Journal Article
Serra, H. - Svačina, Radim - Baumann, U. - Whitford, R. - Sutton, T. - Bartoš, Jan - Sourdille, P.
Ph2 encodes the mismatch repair protein MSH7-3D that inhibits wheat homoeologous recombination.
Nature Communications. Roč. 12, č. 1 (2021), č. článku 803. E-ISSN 2041-1723
R&D Projects: GA ČR(CZ) GA17-05341S; GA MŠMT(CZ) EF16_019/0000827
Institutional support: RVO:61389030
Keywords : hexaploid wheat * DNA Mismatch Repair * Gene Expression
OECD category: Biochemistry and molecular biology
Impact factor: 17.694, year: 2021
Method of publishing: Open access
http://doi.org/10.1038/s41467-021-21127-1

Meiotic recombination is a critical process for plant breeding, as it creates novel allele combinations that can be exploited for crop improvement. In wheat, a complex allohexaploid that has a diploid-like behaviour, meiotic recombination between homoeologous or alien chromosomes is suppressed through the action of several loci. Here, we report positional cloning of Pairing homoeologous 2 (Ph2) and functional validation of the wheat DNA mismatch repair protein MSH7-3D as a key inhibitor of homoeologous recombination, thus solving a half-century-old question. Similar to ph2 mutant phenotype, we show that mutating MSH7-3D induces a substantial increase in homoeologous recombination (up to 5.5 fold) in wheat-wild relative hybrids, which is also associated with a reduction in homologous recombination. These data reveal a role for MSH7-3D in meiotic stabilisation of allopolyploidy and provides an opportunity to improve wheat’s genetic diversity through alien gene introgression, a major bottleneck facing crop improvement.
Permanent Link: http://hdl.handle.net/11104/0322294