Recent Advances in Chiral Analysis of Proteins and Peptides

0545480 - FGÚ 2022 RIV CH eng J - Journal Article
Morvan, Marine - Mikšík, Ivan
Recent Advances in Chiral Analysis of Proteins and Peptides.
Separations. Roč. 8, č. 8 (2021), č. článku 112. E-ISSN 2297-8739
R&D Projects: GA ČR(CZ) GA20-03899S
Institutional support: RVO:67985823
Keywords : chiral separation * proteins * peptides * d-amino acids
OECD category: Analytical chemistry
Impact factor: 3.344, year: 2021
Method of publishing: Open access
https://www.mdpi.com/2297-8739/8/8/112

Like many biological compounds, proteins are found primarily in their homochiral form. However, homochirality is not guaranteed throughout life. Determining their chiral proteinogenic sequence is a complex analytical challenge. This is because certain d-amino acids contained in proteins play a role in human health and disease. This is the case, for example, with d-Asp in elastin, β-amyloid and α-crystallin which, respectively, have an action on arteriosclerosis, Alzheimer’s disease and cataracts. Sequence-dependent and sequence-independent are the two strategies for detecting the presence and position of d-amino acids in proteins. These methods rely on enzymatic digestion by a site-specific enzyme and acid hydrolysis in a deuterium or tritium environment to limit the natural racemization of amino acids. In this review, chromatographic and electrophoretic techniques, such as LC, SFC, GC and CE, will be recently developed (2018–2020) for the enantioseparation of amino acids and peptides. For future work, the discovery and development of new chiral stationary phases and derivatization reagents could increase the resolution of chiral separations.
Permanent Link: http://hdl.handle.net/11104/0322162