Changes of Cerebrospinal Fluid Peptides due to Tauopathy

0545442 - ÚI 2022 NL eng J - Journal Article
Majerová, P. - Barath, P. - Michalicová, A. - Katina, Stanislav - Novák, M. - Kováč, A.
Changes of Cerebrospinal Fluid Peptides due to Tauopathy.
Journal of Alzheimer's Disease. Roč. 58, č. 2 (2017), s. 507-519. ISSN 1387-2877. E-ISSN 1875-8908
Keywords : neuroendocrine protein 7b2 * apolipoprotein-e polymorphism * alzheimers-disease * neurodegenerative diseases * biomarker discovery * multiple-sclerosis * phosphorylated-tau * secretory pathway * axonal-transport * secretogranin-ii * Cerebrospinal fluid * lc-maldi ms * peptidomics * rat model * tauopathy
Impact factor: 3.476, year: 2017

Alzheimer's disease (AD) and progressive supranuclear palsy are two common neurodegenerative tauopathies, and the most common cause of progressive brain dementia in elderly affecting more than 35 million people. The tauopathies are characterized by abnormal deposition of microtubule associated protein tau into intracellular neurofibrillary tangles composed mainly of the hyperphosphorylated form of the protein. The diagnosis of tauopathies is based on the presence of clinical features and pathological changes. Over the last decade, there has been an intensive search for novel biochemical markers for clinical diagnosis of AD and other tauopathies. In the present study, we used transgenic rat model for tauopathy expressing human truncated tau protein (aa 151-391/4R) to analyze the cerebrospinal fluid (CSF) peptidome using liquid chromatography matrix assisted laser desorption/ionization mass spectrometry (LC-MALDI TOF/TOF). From 345 peptides, we identified a total of 175 proteins. Among them, 17 proteins were significantly altered in the CSF of transgenic rats. The following proteins were elevated in the CSF of transgenic rats when compared to the control animals: neurofilament light and medium chain, apolipoprotein E, gamma-synuclein, chromogranin A, reticulon-4, secretogranin-2, calsyntein-1 and3, endothelin-3, neuroendocrine protein B72A, alpha-1-macroglobulin, and augurin. Interestingly most of the identified proteins were previously linked to AD and other tauopathies, indicating the significance of transgenic animals in biomarker validation.
Permanent Link: http://hdl.handle.net/11104/0322128