Progress in forensic bone DNA analysis: Lessons learned from ancient DNA

0545309 - ÚJF 2022 RIV IE eng J - Journal Article
Hofreiter, M. - Šneberger, Jiří - Pospíšek, M. - Vaněk, D.
Progress in forensic bone DNA analysis: Lessons learned from ancient DNA.
Forensic Science International-Genetics. Roč. 54, SEP (2021), č. článku 102538. ISSN 1872-4973. E-ISSN 1878-0326
Institutional support: RVO:61389005
Keywords : Ancient DNA * forensic * inhibitors * degraded DNA
OECD category: Genetics and heredity (medical genetics to be 3)
Impact factor: 4.453, year: 2021
Method of publishing: Limited access
https://doi.org/10.1016/j.fsigen.2021.102538

Research on ancient and forensic DNA is related in many ways, and the two fields must deal with similar ob-stacles. Therefore, communication between these two communities has the potential to improve results in both research fields. Here, we present the insights gained in the ancient DNA community with regard to analyzing DNA from aged skeletal material and the potential use of the developed protocols in forensic work. We discuss the various steps, from choosing samples for DNA extraction to deciding between classical PCR amplification and massively parallel sequencing approaches. Based on the progress made in ancient DNA analyses combined with the requirements of forensic work, we suggest that there is substantial potential for incorporating ancient DNA approaches into forensic protocols, a process that has already begun to a considerable extent. However, taking full advantage of the experiences gained from ancient DNA work will require comparative studies by the forensic DNA community to tailor the methods developed for ancient samples to the specific needs of forensic studies and case work. If successful, in our view, the benefits for both communities would be considerable.
Permanent Link: http://hdl.handle.net/11104/0322037