Direct evidence for crossover and chromatid interference in meiosis of two plant hybrids (Lolium multiflorumxFestuca pratensis and Allium cepaxA. roylei)

0545209 - ÚEB 2022 RIV GB eng J - Journal Article
Ferreira, Marco Tulio Mendes - Glombik, Marek - Perničková, Kateřina - Duchoslav, M. - Scholten, O. - Karafiátová, Miroslava - Techio, V.H. - Doležel, Jaroslav - Lukaszewski, A.J. - Kopecký, David
Direct evidence for crossover and chromatid interference in meiosis of two plant hybrids (Lolium multiflorumxFestuca pratensis and Allium cepaxA. roylei).
Journal of Experimental Botany. Roč. 72, č. 2 (2021), s. 254-267. ISSN 0022-0957. E-ISSN 1460-2431
R&D Projects: GA ČR(CZ) GA20-10019S; GA MŠMT(CZ) EF16_019/0000827
Institutional support: RVO:61389030
Keywords : crossing-over * chiasma interference * meiotic recombination * tetrad analysis * saccharomyces-cerevisiae * individual chromosomes * physical distribution * statistical-analysis * festuca-pratensis * linked genes * Centromere * chromatid interference * crossover interference * homoeologous chromosome * hybrid * meiosis * recombination
OECD category: Genetics and heredity (medical genetics to be 3)
Impact factor: 7.378, year: 2021
Method of publishing: Open access
http://doi.org/10.1093/jxb/eraa455

Crossing over, in addition to its strictly genetic role, also performs a critical mechanical function, by bonding homologues in meiosis. Hence, it is responsible for an orderly reduction of the chromosome number. As such, it is strictly controlled in frequency and distribution. The well-known crossover control is positive crossover interference which reduces the probability of a crossover in the vicinity of an already formed crossover. A poorly studied aspect of the control is chromatid interference. Such analyses are possible in very few organisms as they require observation of all four products of a single meiosis. Here, we provide direct evidence of chromatid interference. Using in situ probing in two interspecific plant hybrids (Lolium multiflorumxFestuca pratensis and Allium cepaxA. roylei) during anaphase I, we demonstrate that the involvement of four chromatids in double crossovers is significantly more frequent than expected (64% versus 25%). We also provide a physical measure of the crossover interference distance, covering similar to 30-40% of the relative chromosome arm length, and show that the centromere acts as a barrier for crossover interference. The two arms of a chromosome appear to act as independent units in the process of crossing over. Chromatid interference has to be seriously addressed in genetic mapping approaches and further studies.
Permanent Link: http://hdl.handle.net/11104/0321945