Parp1 hyperactivity couples DNA breaks to aberrant neuronal calcium signalling and lethal seizures

0544973 - ÚMG 2022 RIV US eng J - Journal Article
Komulainen, E. - Badman, J. - Rey, S. - Rulten, S. - Ju, L. - Fennell, K. - Kalasová, Ilona - Ilievová, Kristýna - McKinnon, P.J. - Hanzlíková, Hana - Staras, K. - Caldecott, Keith
Parp1 hyperactivity couples DNA breaks to aberrant neuronal calcium signalling and lethal seizures.
Embo Reports. Roč. 22, č. 5 (2021), č. článku e51851. ISSN 1469-221X. E-ISSN 1469-3178
Institutional support: RVO:68378050
Keywords : DNA strand break * neurodegeneration * poly(ADP‐ * ribose) polymerase * seizures * xrcc1
OECD category: Biochemistry and molecular biology
Impact factor: 9.421, year: 2021
Method of publishing: Open access
https://www.embopress.org/doi/full/10.15252/embr.202051851

Defects in DNA single-strand break repair (SSBR) are linked with neurological dysfunction but the underlying mechanisms remain poorly understood. Here, we show that hyperactivity of the DNA strand break sensor protein Parp1 in mice in which the central SSBR protein Xrcc1 is conditionally deleted (Xrcc1(Nes-Cre)) results in lethal seizures and shortened lifespan. Using electrophysiological recording and synaptic imaging approaches, we demonstrate that aberrant Parp1 activation triggers seizure-like activity in Xrcc1-defective hippocampus ex vivo and deregulated presynaptic calcium signalling in isolated hippocampal neurons in vitro. Moreover, we show that these defects are prevented by Parp1 inhibition or deletion and, in the case of Parp1 deletion, that the lifespan of Xrcc1(Nes-Cre) mice is greatly extended. This is the first demonstration that lethal seizures can be triggered by aberrant Parp1 activity at unrepaired SSBs, highlighting PARP inhibition as a possible therapeutic approach in hereditary neurological disease.
Permanent Link: http://hdl.handle.net/11104/0321763