Effects of Workers Exposure to Nanoparticles Studied by NMR Metabolomics.

0544564 - ÚCHP 2022 RIV CH eng J - Journal Article
Horník, Štěpán - Michálková, Lenka - Sýkora, Jan - Ždímal, Vladimír - Vlčková, Š. - Dvořáčková, Š. - Pelclová, D.
Effects of Workers Exposure to Nanoparticles Studied by NMR Metabolomics.
Applied Sciences-Basel. Roč. 11, č. 4 (2021), č. článku 6601. E-ISSN 2076-3417
R&D Projects: GA MŠMT(CZ) LM2018122; GA TA ČR(CZ) TK02010035
Institutional support: RVO:67985858
Keywords : exhaled breath condensate * human plasma * nanoparticles exposure * NMR metabolomics
OECD category: Environmental sciences (social aspects to be 5.7)
Impact factor: 2.838, year: 2021
Method of publishing: Open access
https://www.mdpi.com/2076-3417/11/14/6601

In this study, the effects of occupational exposure to nanoparticles (NPs) were studied by NMR metabolomics. Exhaled breath condensate (EBC) and blood plasma samples were obtained from a research nanoparticles-processing unit at a national research university. The samples were taken from three groups of subjects: samples from workers exposed to nanoparticles collected before and after shift, and from controls not exposed to NPs. Altogether, 601H NMR spectra of exhaled breath condensate (EBC) samples and 601H NMR spectra of blood plasma samples were analysed, 20 in each group. The metabolites identified together with binning data were subjected to multivariate statistical analysis, which provided clear discrimination of the groups studied. Statistically sig-nificant metabolites responsible for group separation served as a foundation for analysis of im-paired metabolic pathways. It was found that the acute effect of NPs exposure is mainly reflected in the pathways related to the production of antioxidants and other protective species, while the chronic effect is manifested mainly in the alteration of glutamine and glutamate metabolism, and the purine metabolism pathway.
Permanent Link: http://hdl.handle.net/11104/0321409