Speciation analysis of mercury employing volatile species generation: Approaches to reliable determination in blood and hair

0544232 - ÚIACH 2022 RIV US eng J - Journal Article
Petry-Podgórska, Inga - Schrenková, Věra - Migašová, Michaela - Matoušek, Tomáš - Kratzer, Jan
Speciation analysis of mercury employing volatile species generation: Approaches to reliable determination in blood and hair.
Microchemical Journal. Roč. 170, NOV (2021), č. článku 106606. ISSN 0026-265X. E-ISSN 1095-9149
R&D Projects: GA ČR GA18-01116S
Institutional support: RVO:68081715
Keywords : speciation analysis * human hair * human blood * mercury
OECD category: Analytical chemistry
Impact factor: 5.304, year: 2021
Method of publishing: Limited access

A simple and short (7 min) two-stage extraction procedure was proposed for simultaneous determination of Hg2+, MeHg+ and EtHg+ in human blood. Acceptable extraction efficiency of Hg species without their inter-conversions was confirmed by analysis of certified reference materials resulting in recoveries better than 90% (Hg2+, MeHg+) and 80% for EtHg+, respectively. HPLC separation and following post-column volatile species generation (VSG) steps were optimized to achieve the best analytical performance employing ICP-MS detection. VSG step enhances sensitivity 30–40 times and improves limits of detection (LOD) by a factor of five in comparison with the setup without VSG step. Extraction selectivity of MeHg+ from human hair matrix was verified by HPLC-ICP-MS and applied to both certified reference material and real samples.
Permanent Link: http://hdl.handle.net/11104/0321263