Transformed tissue of Dionaea muscipula J. Ellis as a source of biologically active phenolic compounds with bactericidal properties

0544187 - ÚEB 2022 RIV DE eng J - Journal Article
Makowski, W. - Królicka, A. - Nowicka, Anna - Zwyrtková, Jana - Tokarz, B. - Pečinka, Aleš - Banasiuk, R. - Tokarz, K. M.
Transformed tissue of Dionaea muscipula J. Ellis as a source of biologically active phenolic compounds with bactericidal properties.
Applied Microbiology and Biotechnology. Roč. 105, č. 3 (2021), s. 1215-1226. ISSN 0175-7598. E-ISSN 1432-0614
Institutional support: RVO:61389030
Keywords : Phenolic acids * Plumbagin * Rhizobium rhizogenes * Teratomas * Venus flytrap
OECD category: Biochemistry and molecular biology
Impact factor: 5.560, year: 2021
Method of publishing: Open access
http://doi.org/10.1007/s00253-021-11101-8

The Venus flytrap (Dionaea muscipula J. Ellis) is a carnivorous plant able to synthesize large amounts of phenolic compounds, such as phenylpropanoids, flavonoids, phenolic acids, and 1,4-naphtoquinones. In this study, the first genetic transformation of D. muscipula tissues is presented. Two wild-type Rhizobium rhizogenes strains (LBA 9402 and ATCC 15834) were suitable vector organisms in the transformation process. Transformation led to the formation of teratoma (transformed shoot) cultures with the bacterial rolB gene incorporated into the plant genome in a single copy. Using high-pressure liquid chromatography, we demonstrated that transgenic plants were characterized by an increased quantity of phenolic compounds, including 1,4-naphtoquinone derivative, plumbagin (up to 106.63 mg x g(-1) DW), and phenolic acids (including salicylic, caffeic, and ellagic acid), in comparison to non-transformed plants. Moreover, Rhizobium-mediated transformation highly increased the bactericidal properties of teratoma-derived extracts. The antibacterial properties of transformed plants were increased up to 33% against Staphylococcus aureus, Enterococcus faecalis, and Escherichia coli and up to 7% against Pseudomonas aeruginosa. For the first time, we prove the possibility of D. muscipula transformation. Moreover, we propose that transformation may be a valuable tool for enhancing secondary metabolite production in D. muscipula tissue and to increase bactericidal properties against human antibiotic-resistant bacteria.
Permanent Link: http://hdl.handle.net/11104/0321239