Micellar electrokinetic chromatography in the determination of triazoles in fruit peel

0544131 - ÚOCHB 2022 RIV NL eng J - Journal Article
Kovač, Ishak - Jakl, M. - Šolínová, Veronika - Konášová, Renáta - Kašička, Václav - Jaklová Dytrtová, Jana
Micellar electrokinetic chromatography in the determination of triazoles in fruit peel.
Journal of Chromatography A. Roč. 1652, Aug 30 (2021), č. článku 462385. ISSN 0021-9673. E-ISSN 1873-3778
R&D Projects: GA ČR(CZ) GA18-01710S
Institutional support: RVO:61388963
Keywords : CD-MEKC * tomato peel * penconazole * tebuconazole * cyproconazole * triazoles
OECD category: Analytical chemistry
Impact factor: 4.601, year: 2021
Method of publishing: Limited access
https://doi.org/10.1016/j.chroma.2021.462385

Triazole fungicides (TAFs) are frequently used fungicides for various antifungal treatments of crops. Tre treatment is provided foliarly. However, some significant amount of TAFs may remain on/in fruits. We have developed a methodology for the determination of penconazole, tebuconazole and cyproconazole in tomato fruit peel. The extraction of TAFs was provided with chloroform (acidified with 0.1% acetic acid). In the electrokinetic chromatography, the mixed micellar pseudo-stationary phase was composed of anionic detergent sodium dodecyl sulphate (15 mM) and randomly highly sulphated gamma-cyclodextrin (17.5 mg/mL). The background electrolyte consisted of 100 mM phosphoric acid and 100 mM Tris in the mixed hydro-organic solvent water/methanol (80/20 v/v), apparent pH 4.8. Complete separation of penconazole, tebuconazole, and two diastereomers of cyproconazole with resolutions higher than 5.1 were achieved within a relatively short time of less than 17 min in the bare fused silica capillary of 425/500 mm total/effective lengths and 50/375 μm I.D./O.D. at separation voltage -15 kV (cathode at injection capillary end) and at constant capillary cassette temperature of 22°C. The TAFs were detected by a UV-spectrophotometric diode array detector set at 200 nm. The limits of detection and limits of quantification were in the range of 71-92 and 214–278 μg/kg of peel, respectively. Analyses of the peel extracts revealed that even 10 days after the last treatment, TAF concentrations were higher than the recommended maximum residue limits in both application ways, as individual as well as in the TAF binary or ternary mixtures.
Permanent Link: http://hdl.handle.net/11104/0321175