Side-view holographic endomicroscopy via a custom-terminated multimode fibre

0544059 - ÚPT 2022 RIV US eng J - Journal Article
Silveira, B. M. - Pikálek, Tomáš - Stibůrek, Miroslav - Ondráčková, Petra - Jákl, Petr - Leite, I. T. - Čižmár, Tomáš
Side-view holographic endomicroscopy via a custom-terminated multimode fibre.
Optics Express. Roč. 29, č. 15 (2021), s. 23083-23095. ISSN 1094-4087
R&D Projects: GA MŠMT EF15_003/0000476
Institutional support: RVO:68081731
Keywords : multimode optical fibre * fibre processing * ray optics
OECD category: Optics (including laser optics and quantum optics)
Impact factor: 3.833, year: 2021
Method of publishing: Open access
https://www.osapublishing.org/oe/fulltext.cfm?uri=oe-29-15-23083&id=453102

Microendoscopes based on optical fibres have recently come to the fore as promising candidates allowing in-vivo observations of otherwise inaccessible biological structures in animal models. Despite being still in its infancy, imaging can now be performed at the tip of a single multimode fibre, by relying on powerful holographic methods for light control. Fibre based endoscopy is commonly performed en face, resulting in possible damage of the specimen owing to the direct contact between the distal end of the probe and target. On this ground, we designed an all-fibre probe with an engineered termination that reduces compression and damage to the tissue under investigation upon probe insertion. The geometry of the termination brings the field of view to a plane parallel to the fibre's longitudinal direction, conveying the probe with off-axis imaging capabilities. We show that its focusing ability also benefits from a higher numerical aperture, resulting in imaging with increased spatial resolution. The effect of probe insertion was investigated inside a tissue phantom comprising fluorescent particles suspended in agarose gel, and a comparison was established between the novel side-view probe and the standard en face fibre probe. This new concept paves the way to significantly less invasive deep-tissue imaging.
Permanent Link: http://hdl.handle.net/11104/0321181