Nitrogenous nutrition affects uptake of arsenic and defense enzyme responses in wheat

0544004 - BÚ 2022 RIV PL eng J - Journal Article
Maglovski, M. - Rybanský, L. - Bujdoš, M. - Adamec, Lubomír - Bardáčová, M. - Blehová, A. - Matušíková, I.
Nitrogenous nutrition affects uptake of arsenic and defense enzyme responses in wheat.
Polish Journal of Environmental Studies. Roč. 30, č. 3 (2021), s. 2213-2231. ISSN 1230-1485. E-ISSN 2083-5906
Institutional support: RVO:67985939
Keywords : inducible defense * glucanhydrolases * Triticum aestivum
OECD category: Biochemistry and molecular biology
Impact factor: 1.871, year: 2021
Method of publishing: Open access

Nitrogenous nutrition influences the availability of other plant resources and affects plant defense responses. A series of 8 different NH4NO3 concentrations, ranging from zero to excessive nitrogen (35 mM N), was applied in growth media to hydroponically grown wheat (Triticum aestivum). The plants were grown at a sublethal concentration of arsenic (5 mM As3+) for 10 days and foliar accumulation of As, N and P was determined. Induction of defense-related chitinase and beta-1,3-glucanase enzyme isoforms was quantified upon the separation of plant protein extracts in polyacrylamide gels. As3+ interfered with N and P accumulation in shoots and strongly activated several enzyme isoforms. These responses varied with the N supply and indicated a low rate of As accumulation at low N concentrations. On the other hand, limited As transfer to shoots was a clear benefit at high N concentrations. Nevertheless, both extreme N concentrations restricted the growth. Several enzyme isoforms of both chitinases and beta-1,3-glucanases exerted sensitivity to As3+, N supply or both. Their individual responses, however, contradict the generally accepted view on positive correlation between these defense molecules and N nutrition. Impacts of interplay between As3+ toxicity and nutritional stress on wheat responses are discussed.
Permanent Link: http://hdl.handle.net/11104/0321075