Light and CO2 Modulate the Accumulation and Localization of Phenolic Compounds in Barley Leaves

0543631 - ÚVGZ 2022 RIV CH eng J - Journal Article
Hunt, L. - Klem, Karel - Lhotáková, Z. - Vosolsobě, S. - Oravec, Michal - Urban, Otmar - Špunda, Vladimír - Albrechtová, J.
Light and CO2 Modulate the Accumulation and Localization of Phenolic Compounds in Barley Leaves.
Antioxidants. Roč. 10, č. 3 (2021), č. článku 385. E-ISSN 2076-3921
R&D Projects: GA MŠMT(CZ) EF16_019/0000797; GA ČR(CZ) GA18-23702S
Research Infrastructure: CzeCOS III - 90123
Institutional support: RVO:86652079
Keywords : phenolic compounds * histochemical localization * elevated CO2 * image analysis * irradiance * plant stress * hydroxycinnamic acids * hydroxybenzoic acids * flavonoids * barley
OECD category: Agronomy, plant breeding and plant protection
Impact factor: 7.675, year: 2021
Method of publishing: Open access
https://www.mdpi.com/2076-3921/10/3/385

Barley (Hordeum vulgare) accumulates phenolic compounds (PhCs), which play a key role in plant defense against environmental stressors as antioxidants or UV screening compounds. The influence of light and atmospheric CO2 concentration ([CO2]) on the accumulation and localization of PhCs in barley leaves was examined for two varieties with different tolerances to oxidative stress. PhC localization was visualized in vivo using fluorescence microscopy. Close relationships were found between fluorescence-determined localization of PhCs in barley leaves and PhC content estimated using liquid chromatography coupled with mass spectroscopy detection. Light intensity had the strongest effect on the accumulation of PhCs, but the total PhC content was similar at elevated [CO2], minimizing the differences between high and low light. PhCs localized preferentially near the surfaces of leaves, but under low light, an increasing allocation of PhCs in deeper mesophyll layers was observed. The PhC profile was significantly different between barley varieties. The relatively tolerant variety accumulated significantly more hydroxycinnamic acids, indicating that these PhCs may play a more prominent role in oxidative stress prevention. Our research presents novel evidence that [CO2] modulates the accumulation of PhCs in barley leaves. Mesophyll cells, rather than epidermal cells, were most responsive to environmental stimuli in terms of PhC accumulation.
Permanent Link: http://hdl.handle.net/11104/0320823