Important parameters affecting quality of vitrified donor oocytes

0543575 - BTÚ 2022 RIV US eng J - Journal Article
Teplá, O. - Topurko, Z. - Mašata, J. - Jirsová, S. - Frolíková, Michaela - Komrsková, Kateřina - Minksová, A. - Turánek, J. - Lynnyková, Anna - Kratochvílová, Irena
Important parameters affecting quality of vitrified donor oocytes.
Cryobiology. Roč. 100, JUN 2021 (2021), s. 110-116. ISSN 0011-2240. E-ISSN 1090-2392
R&D Projects: GA TA ČR TK02030069
Grant - others:OP VVV - SOLID21(XE) CZ.02.1.01/0.0/0.0/16_019/0000760
Institutional support: RVO:86652036 ; RVO:68378271
Keywords : Human oocyte vitrification * Oocyte maturation * Stimulated donor oocytes yield
OECD category: Biochemistry and molecular biology; Biophysics (FZU-D)
Impact factor: 2.728, year: 2021
Method of publishing: Open access
https://www.sciencedirect.com/science/article/pii/S0011224021000614

For group of 281 oocytes obtained from 43 stimulated donors and cryopreserved by vitrification protocol using Cryotop and Kitazato medium we determined important parameters of oocytes collection and vitrification processes which strongly affect the probability that warmed oocytes will produce high-quality embryos for transfer. The probability to obtain high-quality embryos for transfer from vitrified and warmed oocytes was highest when two conditions were fulfilled: 1. oocytes were incubated before vitrification for 7-10 h and 2. stimulated ovaries of donors in one cycle produced a smaller number of oocytes (<7 oocytes from one donor per stimulated cycle). The probable reasons for these observations were: 1. early vitrification (less than 7 h) before final oocyte metaphase II maturation negatively affected the crucial process of post-warm remodelling of spindles and chromosomes, which reduced the fertilization and utilization rates, 2. the evaluated vitrification protocol amplifies negative impact of membrane defects of oocytes of those cohorts containing more than 6 oocytes freezing places great demands on the integrity and elasticity of the cell membranes. The fact that cryopreservation influences a complex state of oocytes was confirmed by confocal microscopy.
Permanent Link: http://hdl.handle.net/11104/0320759