A single-cell analytical approach to quantify activated caspase-3/7 during osteoblast proliferation, differentiation, and apoptosis

0543478 - ÚIACH 2022 RIV DE eng J - Journal Article
Killinger, Michael - Veselá, Barbora - Procházková, Markéta - Matalová, Eva - Klepárník, Karel
A single-cell analytical approach to quantify activated caspase-3/7 during osteoblast proliferation, differentiation, and apoptosis.
Analytical and Bioanalytical Chemistry. Roč. 413, č. 20 (2021), s. 5085-5093. ISSN 1618-2642. E-ISSN 1618-2650
R&D Projects: GA ČR(CZ) GA20-00726S
Institutional support: RVO:68081715 ; RVO:67985904
Keywords : apoptosis * differentation * single-cell analysis
OECD category: Analytical chemistry; Developmental biology (UZFG-Y)
Impact factor: 4.478, year: 2021
Method of publishing: Limited access

The protein heterogeneity at the single-cell level has been recognized to be vital for an understanding of various life processes during animal development. In addition, the knowledge of accurate quantity of relevant proteins at cellular level is essential for
appropriate interpretation of diagnostic and therapeutic results. Here, we developed miniaturized instrumentation, and ultrasensitive and highly selective methodology based on bioluminescence photon-counting to quantify activated caspase-3/7 in individual cells.
Permanent Link: http://hdl.handle.net/11104/0320670