Endopolyploidy Variation in Wild Barley Seeds across Environmental Gradients in Israel

0543386 - ÚVGZ 2022 RIV CH eng J - Journal Article
Nowicka, A. - Sahu, Pranav P. - Kovacik, M. - Weigt, D. - Tokarz, B. - Krugman, T. - Pečinka, A.
Endopolyploidy Variation in Wild Barley Seeds across Environmental Gradients in Israel.
Genes. Roč. 12, č. 5 (2021), č. článku 711. E-ISSN 2073-4425
R&D Projects: GA ČR(CZ) GJ20-25845Y
Institutional support: RVO:86652079
Keywords : programmed cell-death * hordeum-spontaneum * domestication history * phenotypic diversity * genetic diversity * ssp spontaneum * endoreduplication * endosperm * arabidopsis * growth * Endoreduplication * endosperm * Hordeum vulgare ubsp * spontaneum * seed development * super cycle value
OECD category: Genetics and heredity (medical genetics to be 3)
Impact factor: 4.141, year: 2021
Method of publishing: Open access
https://www.mdpi.com/2073-4425/12/5/711

Wild barley is abundant, occupying large diversity of sites, ranging from the northern mesic Mediterranean meadows to the southern xeric deserts in Israel. This is also reflected in its wide phenotypic heterogeneity. We investigated the dynamics of DNA content changes in seed tissues in ten wild barley accessions that originated from an environmental gradient in Israel. The flow cytometric measurements were done from the time shortly after pollination up to the dry seeds. We show variation in mitotic cell cycle and endoreduplication dynamics in both diploid seed tissues (represented by seed maternal tissues and embryo) and in the triploid endosperm. We found that wild barley accessions collected at harsher xeric environmental conditions produce higher proportion of endoreduplicated nuclei in endosperm tissues. Also, a comparison of wild and cultivated barley strains revealed a higher endopolyploidy level in the endosperm of wild barley, that is accompanied by temporal changes in the timing of the major developmental phases. In summary, we present a new direction of research focusing on connecting spatiotemporal patterns of endoreduplication in barley seeds and possibly buffering for stress conditions.
Permanent Link: http://hdl.handle.net/11104/0320600