Interaction of Tris with DNA molecules and carboxylic groups on self-assembled monolayers of alkanethiols measured with surface plasmon resonance

0543285 - ÚFE 2022 RIV NL eng J - Journal Article
Šípová, Hana - Jurgová, Ludmila - Hemmerová, Erika - Homola, Jiří
Interaction of Tris with DNA molecules and carboxylic groups on self-assembled monolayers of alkanethiols measured with surface plasmon resonance.
Applied Surface Science. Roč. 546, APR (2021), č. článku 148984. ISSN 0169-4332. E-ISSN 1873-5584
R&D Projects: GA ČR(CZ) GA19-02739S
Institutional support: RVO:67985882
Keywords : Surface plasmon resonance * Biosensor * Biomolecular interactions * Tris buffer
OECD category: Atomic, molecular and chemical physics (physics of atoms and molecules including collision, interaction with radiation, magnetic resonances, Mössbauer effect)
Impact factor: 7.392, year: 2021
Method of publishing: Limited access
https://doi.org/10.1016/j.apsusc.2021.148984

Functional materials employing organic coatings on inorganic substrates are perceived as potential platforms for applications in a variety of fields. Therefore, the investigation of interactions of such systems with the micro-environment has become an important research direction in surface science. Herein, we study the interaction of one of the buffers most commonly used in biological studies, Tris buffer, with self-assembled monolayers (SAMs) of alkanethiols and short DNAs using a surface plasmon resonance (SPR) biosensor. We show that the interaction between Tris and carboxylic groups of SAMs is a complex multiphasic process. We demonstrate that Tris base binds to the protonated carboxylic groups. When those groups become deprotonated, Tris base dissociates and Tris acid is attracted, which results in the formation of a diffuse layer over the charged surface. In addition, we show that the interaction of Tris with the immobilized DNA molecules biases the determination of surface concentrations of the immobilized DNA molecules and thus also the determination of hybridization efficiencies
Permanent Link: http://hdl.handle.net/11104/0320518