Thermo- and ROS-responsive self-assembled polymer nanoparticle tracers for 19F MRI theranostics

0543182 - ÚMCH 2022 RIV US eng J - Journal Article
Kolouchová, Kristýna - Groborz, Ondřej - Černochová, Zulfiya - Škarková, A. - Brabek, J. - Rosel, D. - Švec, Pavel - Starčuk, Zenon - Šlouf, Miroslav - Hrubý, Martin
Thermo- and ROS-responsive self-assembled polymer nanoparticle tracers for 19F MRI theranostics.
Biomacromolecules. Roč. 22, č. 6 (2021), s. 2325-2337. ISSN 1525-7797. E-ISSN 1526-4602
R&D Projects: GA MŠMT(CZ) LTC19032; GA MŠMT(CZ) LM2018133; GA ČR(CZ) GA19-01438S; GA MŠMT(CZ) LM2018129
Grant - others:AV ČR(CZ) StrategieAV21/6; AV ČR(CZ) StrategieAV21/10
Program: StrategieAV; StrategieAV
Research Infrastructure: EATRIS-CZ III - 90133; Czech-BioImaging II - 90129
Institutional support: RVO:61389013 ; RVO:68081731
Keywords : thermoresponsivity * redox responsivity * reactive oxygen species
OECD category: Polymer science; Medical laboratory technology (including laboratory samples analysis (UPT-D)
Impact factor: 6.979, year: 2021
Method of publishing: Limited access
https://pubs.acs.org/doi/10.1021/acs.biomac.0c01316

Fluorine-19 magnetic resonance imaging (19F MRI) enables detailed in vivo tracking of fluorine-containing tracers and is therefore becoming a particularly useful tool in noninvasive medical imaging. In previous studies, we introduced biocompatible polymers based on the hydrophilic monomer N-(2-hydroxypropyl)methacrylamide (HPMA) and the thermoresponsive monomer N-(2,2-difluoroethyl)acrylamide (DFEA). These polymers have abundant magnetically equivalent fluorine atoms and advantageous properties as 19F MRI tracers. Furthermore, in this pilot study, we modified these polymers by introducing a redox-responsive monomer. As a result, our polymers changed their physicochemical properties once exposed to an oxidative environment. Reactive oxygen species (ROS)-responsive polymers were prepared by incorporating small amounts (0.9–4.5 mol %) of the N-[2-(ferrocenylcarboxamido)ethyl]acrylamide (FcCEA) monomer, which is hydrophobic and diamagnetic in the reduced electroneutral (Fe(II), ferrocene) state but hydrophilic and paramagnetic in the oxidized (Fe(III), ferrocenium cation) state. This property can be useful for theranostic purposes (therapy and diagnostic purposes), especially, in terms of ROS-responsive drug-delivery systems. In the reduced state, these nanoparticles remain self-assembled with the encapsulated drug but release the drug upon oxidation in ROS-rich tumors or inflamed tissues.
Permanent Link: http://hdl.handle.net/11104/0320973