0543177 - MBÚ 2022 RIV CH eng J - Journal Article
Buchroithner, B. - Spurný, Pavel - Mayr, S. - Heitz, J. - Sivun, D. - Jacak, J. - Ludwig, Jost
An Improved Transwell Design for Microelectrode Ion-Flux Measurements.
Micromachines. Roč. 12, č. 3 (2021), č. článku 273. ISSN 2072-666X. E-ISSN 2072-666X
EU Projects: European Commission ATCZ14
Research Infrastructure: CENAKVA - 90099
Institutional support: RVO:61388971
Keywords : multiphoton lithography * biocompatible polymers * BisSR * HUVECs * mife * ion flux
OECD category: Biophysics
Impact factor: 3.523, year: 2021
Method of publishing: Open access
Result website:
https://www.mdpi.com/2072-666X/12/3/273
DOI: https://doi.org/10.3390/mi12030273

The microelectrode ion flux estimation (MIFE) is a powerful, non-invasive electrophysiological method for cellular membrane transport studies. Usually, the MIFE measurements are performed in a tissue culture dish or directly with tissues (roots, parts of the plants, and cell tissues). Here, we present a transwell system that allows for MIFE measurements on a cell monolayer. We introduce a measurement window in the transwell insert membrane, which provides direct access for the cells to the media in the upper and lower compartment of the transwell system and allows direct cell-to-cell contact coculture. Three-dimensional multiphoton lithography (MPL) was used to construct a 3D grid structure for cell support in the measurement window. The optimal polymer grid constant was found for implementation in transwell MIFE measurements. We showed that human umbilical vein endothelial cells (HUVECs) efficiently grow and maintain their physiological response on top of the polymer structures.

Permanent Link: http://hdl.handle.net/11104/0320450