Number of the records: 1
Large-scale comparative analysis of cytogenetic markers across Lepidoptera
- 1.0543142 - BC 2022 RIV GB eng J - Journal Article
Provazníková, Irena - Hejníčková, Martina - Visser, Sander - Dalíková, Martina - Carabajal Paladino, L. Z. - Zrzavá, Magda - Voleníková, Anna - Marec, František - Nguyen, Petr
Large-scale comparative analysis of cytogenetic markers across Lepidoptera.
Scientific Reports. Roč. 11, č. 1 (2021), č. článku 12214. ISSN 2045-2322. E-ISSN 2045-2322
R&D Projects: GA ČR(CZ) GA17-13713S; GA ČR(CZ) GA20-13784S
Grant - others:GA ČR(CZ) GA17-17211S
Institutional support: RVO:60077344
Keywords : chromosomal mapping * repetitive DNA sequences * moths and butterflies
OECD category: Genetics and heredity (medical genetics to be 3)
Impact factor: 4.997, year: 2021
Method of publishing: Open access
https://www.nature.com/articles/s41598-021-91665-7.pdf
Fluorescence in situ hybridization (FISH) allows identification of particular chromosomes and their rearrangements. Using FISH with signal enhancement via antibody amplification and enzymatically catalysed reporter deposition, we evaluated applicability of universal cytogenetic markers, namely 18S and 5S rDNA genes, U1 and U2 snRNA genes, and histone H3 genes, in the study of the karyotype evolution in moths and butterflies. Major rDNA underwent rather erratic evolution, which does not always reflect chromosomal changes. In contrast, the hybridization pattern of histone H3 genes was well conserved, reflecting the stable organisation of lepidopteran genomes. Unlike 5S rDNA and U1 and U2 snRNA genes which we failed to detect, except for 5S rDNA in a few representatives of early diverging lepidopteran lineages. To explain the negative FISH results, we used quantitative PCR and Southern hybridization to estimate the copy number and organization of the studied genes in selected species. The results suggested that their detection was hampered by long spacers between the genes and/or their scattered distribution. Our results question homology of 5S rDNA and U1 and U2 snRNA loci in comparative studies. We recommend the use of histone H3 in studies of karyotype evolution.
Permanent Link: http://hdl.handle.net/11104/0326532
Number of the records: 1