Influence of silymarin components on keratinocytes and 3D reconstructed epidermis

0542993 - MBÚ 2022 RIV GB eng J - Journal Article
Franková, J. - Juráňová, J. - Biedermann, David - Ulrichová, J.
Influence of silymarin components on keratinocytes and 3D reconstructed epidermis.
Toxicology in Vitro. Roč. 74, AUG 2021 (2021), č. článku 105162. ISSN 0887-2333. E-ISSN 1879-3177
R&D Projects: GA ČR(CZ) GA18-00132S
Institutional support: RVO:61388971
Keywords : Keratinocytes * rhe * Inflammation * Silymarin
OECD category: Toxicology
Impact factor: 3.685, year: 2021
Method of publishing: Limited access
https://www.sciencedirect.com/science/article/pii/S0887233321000874

Silymarin is a flavonoid complex isolated from the plant Silybum marianum which is well known for its antioxidant, hepatoprotective and immunomodulatory effects. Since little is known about its anti-inflammatory properties and healing effects, our study focused on whether or not silymarin components reduce inflammation and support epidermis regeneration. Lipopolysaccharides (LPS) and sodium dodecyl sulfate (SDS) were used to induce inflammation in normal human epidermal keratinocytes (NHEKs) and reconstructed epidermis (RHE), respectively. The expression of pro-inflammatory cytokines (IL-1, IL-6 and IL-8) in NHEKs and RHE was measured by enzyme linked immunosorbent assay (ELISA). The expression of cytokeratin 14 and loricrin in RHE was detected by immunofluorescent analysis. Hematoxylin and eosin staining was used for the morphological evaluation of RHE. It was determined that 2, 3 dehydrosilybin (DHSB) downregulated the production of selected pro-inflammatory cytokines produced by NHEKs. Although all layers of RHE displayed full thickness, when SDS was applied, cell detachment was seen in the stratum corneum and loricrin expression was diminished.
Permanent Link: http://hdl.handle.net/11104/0320317