Modification of heat-induced whey protein isolate hydrogel with highly bioactive glass particles results in promising biomaterial for bone tissue engineering

0542895 - FGÚ 2022 RIV GB eng J - Journal Article
Dziadek, M. - Charuza, K. - Kudláčková, Radmila - Aveyard, J. - D'Sa, R. - Serafim, A. - Stancu, I.-C. - Iovu, H. - Kerns, J. G. - Allinson, S. - Dziadek, K. - Szatkowski, P. - Cholewa-Kowalska, K. - Bačáková, Lucie - Pamula, E. - Douglas, T. E. L.
Modification of heat-induced whey protein isolate hydrogel with highly bioactive glass particles results in promising biomaterial for bone tissue engineering.
Materials and Design. Roč. 205, July (2021), č. článku 109749. ISSN 0264-1275. E-ISSN 1873-4197
R&D Projects: GA ČR(CZ) GA20-01570S
Institutional support: RVO:67985823
Keywords : waste material * mineralization * enzymatic degradation * antioxidant activity * dynamic mechanical analysis * micro-computed tomography
OECD category: Technologies involving the manipulation of cells, tissues, organs or the whole organism (assisted reproduction)
Impact factor: 9.417, year: 2021
Method of publishing: Open access
https://doi.org/10.1016/j.matdes.2021.109749

This study deals with the design and comprehensive evaluation of novel hydrogels based on whey protein isolate (WPI) for tissue regeneration. So far, WPI has been considered mainly as a food industry by-product and there are very few reports on the application of WPI in tissue engineering (TE). In this work, WPI-based hydrogels were modified with bioactive glass (BG), which is commonly used as a bone substitute material. Ready-to-use, sterile hydrogels were produced by a simple technique, namely heat-induced gelation. Two different concentrations (10 and 20% w/w) of sol–gel-derived BG particles of two different sizes (2.5 and <45 µm) were compared. µCT analysis showed that hydrogels were highly porous with almost 100% pore interconnectivity. BG particles were generally homogenously distributed in the hydrogel matrix, affecting pore size, and reducing material porosity. Thermal analysis showed that the presence of BG particles in WPI matrix reduced water content in hydrogels and improved their thermal stability. BG particles decreased enzymatic degradation of the materials. The materials underwent mineralization in simulated biological fluids (PBS and SBF) and possessed high radical scavenging capacity. In vitro tests indicated that hydrogels were cytocompatible and supported MG-63 osteoblastic cell functions.
Permanent Link: http://hdl.handle.net/11104/0320234