Cytokinin n-glucosides: Occurrence, metabolism and biological activities in plants

0542844 - ÚEB 2022 RIV CH eng J - Journal Article
Pokorná, Eva - Hluska, Tomáš - Galuszka, P. - Hallmark, H. T. - Dobrev, Petre - Záveská Drábková, Lenka - Filipi, T. - Holubová, K. - Plíhal, Ondřej - Rashotte, A.M. - Filepová, Roberta - Malbeck, Jiří - Novák, Ondřej - Spíchal, L. - Brzobohatý, Břetislav - Mazura, P. - Zahajská, Lenka - Motyka, Václav
Cytokinin n-glucosides: Occurrence, metabolism and biological activities in plants.
Biomolecules. Roč. 11, č. 1 (2021), č. článku 24. E-ISSN 2218-273X
R&D Projects: GA ČR(CZ) GA19-12262S; GA ČR GA19-13103S; GA ČR(CZ) GA19-02699S; GA MŠMT(CZ) EF16_019/0000738; GA MŠMT(CZ) LQ1601
Institutional support: RVO:61389030 ; RVO:68081707
Keywords : Arabidopsis * Cis-zeatin * Cytokinin * Cytokinin oxidase/dehydrogenase * N-glucosides * Oat * Senescence * Trans-zeatin * Zea mays * [2-3H]tZ9G 3 * β-D-glucosidase Zm-p60.1
OECD category: Plant sciences, botany
Impact factor: 6.064, year: 2021
Method of publishing: Open access
http://doi.org/10.3390/biom11010024

Cytokinins (CKs) are a class of phytohormones affecting many aspects of plant growth and development. In the complex process of CK homeostasis in plants, N-glucosylation represents one of the essential metabolic pathways. Its products, CK N7-and N9-glucosides, have been largely overlooked in the past as irreversible and inactive CK products lacking any relevant physiological impact. In this work, we report a widespread distribution of CK N-glucosides across the plant kingdom proceeding from evolutionary older to younger plants with different proportions between N7-and N9-glucosides in the total CK pool. We show dramatic changes in their profiles as well as in expression levels of the UGT76C1 and UGT76C2 genes during Arabidopsis ontogenesis. We also demonstrate specific physiological effects of CK N-glucosides in CK bioassays including their antisenescent activities, inhibitory effects on root development, and activation of the CK signaling pathway visualized by the CK-responsive YFP reporter line, TCSv2::3XVENUS. Last but not least, we present the considerable impact of CK N7-and N9-glucosides on the expression of CK-related genes in maize and their stimulatory effects on CK oxidase/dehydrogenase activity in oats. Our findings revise the apparent irreversibility and inactivity of CK N7-and N9-glucosides and indicate their involvement in CK evolution while suggesting their unique function(s) in plants.
Permanent Link: http://hdl.handle.net/11104/0320185