In Vitro Polyploidization of Thymus vulgaris L. and Its Effect on Composition of Essential Oils

0542600 - ÚVGZ 2022 RIV CH eng J - Journal Article
Navrátilová, B. - Švécarová, M. - Bednář, Jan - Ondřej, V.
In Vitro Polyploidization of Thymus vulgaris L. and Its Effect on Composition of Essential Oils.
Agronomy. Roč. 11, č. 3 (2021), č. článku 596. E-ISSN 2073-4395
R&D Projects: GA MŠMT(CZ) LO1415; GA MZe(CZ) QK1910103
Research Infrastructure: CzeCOS III - 90123
Institutional support: RVO:86652079
Keywords : botanical insecticides * chromosome doubling * essential oils * micropropagation * tetraploids * thyme
OECD category: Agriculture
Impact factor: 3.949, year: 2021
Method of publishing: Open access
https://www.mdpi.com/2073-4395/11/3/596

The aim of this work was to find an effective protocol for in vitro propagation and to perform the in vitro polyploidization of diploid Thymus vulgaris (2n = 30) using two experimental methods based on the use of oryzalin, an antimitotic agent. The ploidy level of the obtained shoots was checked by flow cytometric analysis. The most efficient conditions for inducing polyploidy were oryzalin concentrations of 0.346 and 1.73 mg L-1 present in the medium for two weeks. The vital polyploid shoots were multiplied for further evaluation, rooting and final transfer to nonsterile glasshouse and field conditions. The chemical compositions of the essential oils (EOs)-which were obtained from dried field grown plants by steam distillation-were analyzed by gas chromatography/mass spectrometry (GC/MS). The identified substances contributed approximately 95% to the total peak area. Statistical analysis revealed that the tetraploid subclone and the diploid reference plant do not differ in total terpene content, but they do differ in the relative proportions of all the individual terpenes with the exception of alpha-pinene and UN5, indicating that both clones produce EOs of different quality. The obtained results showed the possibility of developing more efficient botanical insecticides based on EOs obtained from the tetraploid plants.
Permanent Link: http://hdl.handle.net/11104/0319985