Complex Response of the Chlorarachniophyte Bigelowiella natans to Iron Availability

0542515 - MBÚ 2022 RIV GB eng J - Journal Article
Kotabová, Eva - Malych, R. - Karlusich, J. J. P. - Kazamia, E. - Eichner, Meri - Mach, J. - Lesuisse, E. - Bowler, Ch. - Prášil, Ondřej - Suták, R.
Complex Response of the Chlorarachniophyte Bigelowiella natans to Iron Availability.
mSystems. Roč. 6, č. 1 (2021), č. článku e00738-20. ISSN 2379-5077. E-ISSN 2379-5077
R&D Projects: GA ČR(CZ) GA18-07822S; GA MŠMT(CZ) LQ1604
Institutional support: RVO:61388971
Keywords : Bigelowiella natans * iron * metagenomics * metatranscriptomics * photosynthesis * phytoplankton * proteomics
OECD category: Microbiology
Impact factor: 7.328, year: 2021
Method of publishing: Open access
https://msystems.asm.org/content/6/1/e00738-20.abstract

The productivity of the ocean is largely dependent on iron availability, and marine phytoplankton have evolved sophisticated mechanisms to cope with chronically low iron levels in vast regions of the open ocean. By analyzing the metabarcoding data generated from the Tara Oceans expedition, we determined how the global distribution of the model marine chlorarachniophyte Bigelowiella natans varies across regions with different iron concentrations. We performed a comprehensive proteomics analysis of the molecular mechanisms underpinning the adaptation of B. natans to iron scarcity and report on the temporal response of cells to iron enrichment. Our results highlight the role of phytotransferrin in iron homeostasis and indicate the involvement of CREG1 protein in the response to iron availability. Analysis of the Tara Oceans metagenomes and metatranscriptomes also points to a similar role for CREG1, which is found to be widely distributed among marine plankton but to show a strong bias in gene and transcript abundance toward iron-deficient regions. Our analyses allowed us to define a new subfamily of the CobW domain-containing COG0523 putative metal chaperones which are involved in iron metabolism and are restricted to only a few phytoplankton lineages in addition to B. natans. At the physiological level, we elucidated the mechanisms allowing a fast recovery of PSII photo-chemistry after resupply of iron. Collectively, our study demonstrates that B. natans is well adapted to dynamically respond to a changing iron environment and suggests that CREG1 and COG0523 are important components of iron homeostasis in B. natans and other phytoplankton.
Permanent Link: http://hdl.handle.net/11104/0319911