Metabolomic characterisation of progression and spontaneous regression of melanoma in the melanoma-bearing Libechov minipig model

0542473 - ÚŽFG 2022 RIV GB eng J - Journal Article
Kertys, M. - Grendar, M. - Horák, Vratislav - Zideková, N. - Kupcová Skalníková, Helena - Mokrý, J. - Halašová, E. - Strnádel, J.
Metabolomic characterisation of progression and spontaneous regression of melanoma in the melanoma-bearing Libechov minipig model.
Melanoma Research. Roč. 31, č. 2 (2021), s. 140-151. ISSN 0960-8931. E-ISSN 1473-5636
R&D Projects: GA MŠMT(CZ) LO1609; GA MŠMT(CZ) EF16_019/0000785
Institutional support: RVO:67985904
Keywords : LC-MS * melanoma * melanoma-bearing Libechov minipig
OECD category: Oncology
Impact factor: 3.199, year: 2021
Method of publishing: Limited access
https://asep.lib.cas.cz/arl-cav/cs/csg/?repo=crepo1&key=73032377889

Melanoma-bearing Libechov minipig (MeLiM) represents a large animal model for melanoma research. This model shows a high incidence of complete spontaneous regression of melanoma - a phenomenon uncommon in humans. Here, we present the first metabolomic characterisation of the MeLiM model comparing animals with progressing and spontaneously regressing melanomas. Plasma samples of 19 minipigs with progression and 27 minipigs with evidence of regression were analysed by a targeted metabolomic assay based on mass spectrometry detection. Differences in plasma metabolomics patterns were investigated by univariate and multivariate statistical analyses. Overall, 185 metabolites were quantified in each plasma sample. Significantly altered metabolomic profile was found, and 42 features were differentially regulated in plasma. Besides, the machine learning approach was used to create a predictive model utilising Arg/Orn and Arg/ADMA ratios to discriminate minipigs with progressive disease development from minipigs with regression evidence. Our results suggest that progression of melanoma in the MeLiM model is associated with alteration of arginine, glycerophospholipid and acylcarnitines metabolism. Moreover, this study provides targeted metabolomics characterisation of an animal model of melanoma with progression and spontaneous regression of tumours.
Permanent Link: http://hdl.handle.net/11104/0319877