The Effect of the Controlled Release of Platelet Lysate from PVA Nanomats on Keratinocytes, Endothelial Cells and Fibroblasts

0542427 - FGÚ 2022 RIV CH eng J - Journal Article
Filová, Elena - Blanquer, Andreu - Knitlová, Jarmila - Plencner, Martin - Jenčová, V. - Kopřivová, B. - Lisnenko, M. - Kuželová Košťáková, E. - Procházková, R. - Bačáková, Lucie
The Effect of the Controlled Release of Platelet Lysate from PVA Nanomats on Keratinocytes, Endothelial Cells and Fibroblasts.
Nanomaterials. Roč. 11, č. 4 (2021), č. článku 995. E-ISSN 2079-4991
R&D Projects: GA MZd(CZ) NV18-01-00332
Institutional support: RVO:67985823
Keywords : controlled release * platelet lysate * PVA nanofibers * endothelial cells * keratinocytes * fibroblasts * cell differentiation
OECD category: Endocrinology and metabolism (including diabetes, hormones)
Impact factor: 5.719, year: 2021
Method of publishing: Open access
https://www.mdpi.com/2079-4991/11/4/995

Platelet lysate (PL) provides a natural source of growth factors and other bioactive molecules, and the local controlled release of these bioactive PL components is capable of improving the healing of chronic wounds. Therefore, we prepared composite nanofibrous meshes via the needleless electrospinning technique using poly(vinyl alcohol) (PVA) with a high molecular weight and with a high degree of hydrolysis with the incorporated PL (10% w/w). The morphology, wettability and protein release from the nanofibers was then assessed from the resulting composite PVA-PL nanomats. The bioactivity of the PVA-PL nanomats was proved in vitro using HaCaT keratinocytes, human saphenous endothelial cells (HSVECs) and 3T3 fibroblasts. The PVA-PL supported cell adhesion, proliferation, and viability. The improved phenotypic maturation of the HaCaT cells due to the PVA-PL was manifested via the formation of intermediate filaments positive for cytokeratin 10. The PVA-PL enhanced both the synthesis of the von Willebrand factor via HSVECs and HSVECs chemotaxis through membranes with 8 mu m-sized pores. These results indicated the favorable effects of the PVA-PL nanomats on the three cell types involved in the wound healing process, and established PVA-PL nanomats as a promising candidate for further evaluation with respect to in vivo experiments.
Permanent Link: http://hdl.handle.net/11104/0319837