Fluorophore Multimerization as an Efficient Approach towards Bright Protein Labels

0542326 - ÚOCHB 2022 RIV DE eng J - Journal Article
Reiber, T. - Zavoiura, O. - Dose, C. - Yushchenko, Dmytro A.
Fluorophore Multimerization as an Efficient Approach towards Bright Protein Labels.
European Journal of Organic Chemistry. Roč. 2021, č. 20 (2021), s. 2817-2830. ISSN 1434-193X. E-ISSN 1099-0690
Institutional support: RVO:61388963
Keywords : flow cytometry * fluorescence microscopy * fluorophore * multimers * protein labelling
OECD category: Organic chemistry
Impact factor: 3.261, year: 2021
Method of publishing: Limited access
https://doi.org/10.1002/ejoc.202100117

Cell analysis techniques like flow cytometry and fluorescence microscopy are widely used to explore cells in real‐time and provide important insights into a variety of cellular processes. These techniques require bright and specific staining reagents to generate strong fluorescence signal and enable reliable read‐out, making the choice of fluorescent labels a key aspect of experimental design. Much research has been done in the field of fluorophore development to generate bright small‐molecule dyes, fluorescent proteins, and emitting nanoparticles. However, it remains challenging to modify biomolecules with multiple emitters and avoid self‐quenching of such fluorophores at the same time. Herein, we present advances in multimerization‐based methods for the generation of fluorescent labels with high brightness and discuss their advantages and limitations in the context of flow cytometry and fluorescence microscopy applications.
Permanent Link: http://hdl.handle.net/11104/0319759