gamma-Tubulin Complexes and Fibrillar Arrays: Two Conserved High Molecular Forms with Many Cellular Functions

0542216 - MBÚ 2022 RIV CH eng J - Journal Article
Chumová, Jana - Kourová, Hana - Trögelová, Lucie - Daniel, G. - Binarová, Pavla
gamma-Tubulin Complexes and Fibrillar Arrays: Two Conserved High Molecular Forms with Many Cellular Functions.
Cells. Roč. 10, č. 4 (2021), č. článku 776. E-ISSN 2073-4409
Grant - others:AV ČR(CZ) MSM200201901
Program: Program na podporu mezinárodní spolupráce začínajících výzkumných pracovníků
Institutional support: RVO:61388971
Keywords : microtubules * plants * gamma-tubulin * gamma-tubulin complexes * fibrillar arrays * nucleation * signaling * sequestration
OECD category: Cell biology
Impact factor: 7.666, year: 2021
Method of publishing: Open access
https://www.mdpi.com/2073-4409/10/4/776

Higher plants represent a large group of eukaryotes where centrosomes are absent. The functions of gamma-tubulin small complexes (gamma-TuSCs) and gamma-tubulin ring complexes (gamma-TuRCs) in metazoans and fungi in microtubule nucleation are well established and the majority of components found in the complexes are present in plants. However, plant microtubules are also nucleated in a gamma-tubulin-dependent but gamma-TuRC-independent manner. There is growing evidence that gamma-tubulin is a microtubule nucleator without being complexed in gamma-TuRC. Fibrillar arrays of gamma-tubulin were demonstrated in plant and animal cells and the ability of gamma-tubulin to assemble into linear oligomers/polymers was confirmed in vitro for both native and recombinant gamma-tubulin. The functions of gamma-tubulin as a template for microtubule nucleation or in promoting spontaneous nucleation is outlined. Higher plants represent an excellent model for studies on the role of gamma-tubulin in nucleation due to their acentrosomal nature and high abundancy and conservation of gamma-tubulin including its intrinsic ability to assemble filaments. The defining scaffolding or sequestration functions of plant gamma-tubulin in microtubule organization or in nuclear processes will help our understanding of its cellular roles in eukaryotes.
Permanent Link: http://hdl.handle.net/11104/0319687