Treatment With Resveratrol Ameliorates Mitochondrial Dysfunction During the Acute Phase of Status Epilepticus in Immature Rats

0542136 - FGÚ 2022 RIV CH eng J - Journal Article
Folbergrová, Jaroslava - Ješina, Pavel - Otáhal, Jakub
Treatment With Resveratrol Ameliorates Mitochondrial Dysfunction During the Acute Phase of Status Epilepticus in Immature Rats.
Frontiers in Neuroscience. Roč. 15, Mar 5 (2021), č. článku 634378. E-ISSN 1662-453X
R&D Projects: GA ČR(CZ) GA15-08565S; GA ČR(CZ) GA18-07908S
Grant - others:AV ČR(CZ) StrategieAV21/7
Program: StrategieAV
Institutional support: RVO:67985823
Keywords : reactive oxygen species * immature rats * status epilepticus * superoxide anion production * deficiency of mitochondrial complex I activity * resveratrol * protection
OECD category: Neurosciences (including psychophysiology
Impact factor: 5.152, year: 2021
Method of publishing: Open access
https://doi.org/10.3389/fnins.2021.634378

The aim of the present study was to elucidate the effect of resveratrol (natural polyphenol) on seizure activity, production of ROS, brain damage and mitochondrial function in the early phase of status epilepticus (SE), induced in immature 12 day-old rats by substances of a different mechanism of action (Li-pilocarpine, DL-homocysteic acid, 4-amino pyridine, and kainate). Seizure activity, production of superoxide anion, brain damage and mitochondrial function were assessed by EEG recordings, hydroethidium method, FluoroJadeB staining and Complex I activity measurement. A marked decrease of complex I activity associated with the acute phase of SE in immature brain was significantly attenuated by resveratrol, given i.p. in two or three doses (25 mg/kg each), 30 min before, 30 or 30 and 60 min after the induction of SE. Increased O-2 center dot(-) production was completely normalized, brain damage partially attenuated. Since resveratrol did not influence seizure activity itself (latency, intensity, frequency), the mechanism of protection is likely due to its antioxidative properties. The findings have a clinical relevance, suggesting that clinically available substances with antioxidant properties might provide a high benefit as an add-on therapy during the acute phase of SE, influencing also mechanisms involved in the development of epilepsy.
Permanent Link: http://hdl.handle.net/11104/0319622