The intestinal microbiota and metabolites in patients with anorexia nervosa

0542104 - MBÚ 2022 RIV US eng J - Journal Article
Procházková, Petra - Roubalová, Radka - Dvořák, Jiří - Kreisinger, J. - Hill, M. - Tlaskalová-Hogenová, Helena - Tomášová, Petra - Pelantová, Helena - Čermáková, Martina - Kuzma, Marek - Bulant, J. - Bilej, Martin - Smitka, K. - Lambertová, A. - Holanová, P. - Papežová, H.
The intestinal microbiota and metabolites in patients with anorexia nervosa.
Gut Microbes. Roč. 13, č. 1 (2021), č. článku e1902771. ISSN 1949-0976. E-ISSN 1949-0984
R&D Projects: GA MZd(CZ) NV17-28905A; GA MZd(CZ) NV18-01-00040
Institutional support: RVO:61388971
Keywords : Microbiome * bacteriome * mycobiome * scfa * neurotransmitter * ede-q * bmi * dysbiosis * renourishment * gut-brain-microbiota axis
OECD category: Microbiology
Impact factor: 9.434, year: 2021
Method of publishing: Open access
https://www.tandfonline.com/doi/full/10.1080/19490976.2021.1902771

Brain-gut microbiota interactions are intensively studied in connection with various neurological and psychiatric diseases. While anorexia nervosa (AN) pathophysiology is not entirely clear, it is presumably linked to microbiome dysbiosis. We aimed to elucidate the gut microbiota contribution in AN disease pathophysiology. We analyzed the composition and diversity of the gut microbiome of patients with AN (bacteriome and mycobiome) from stool samples before and after renourishment, and compared them to healthy controls. Further, levels of assorted neurotransmitters and short-chain fatty acids (SCFA) were analyzed in stool samples by MS and NMR, respectively. Biochemical, anthropometric, and psychometric profiles were assessed. The bacterial alpha-diversity parameter analyses revealed only increased Chao 1 index in patients with AN before the realimentation, reflecting their interindividual variation. Subsequently, core microbiota depletion signs were observed in patients with AN. Overrepresented OTUs (operation taxonomic units) in patients with AN taxonomically belonged to Alistipes, Clostridiales, Christensenellaceae, and Ruminococcaceae. Underrepresented OTUs in patients with AN were Faecalibacterium, Agathobacter, Bacteroides, Blautia, and Lachnospira. Patients exhibited greater interindividual variation in the gut bacteriome, as well as in metagenome content compared to controls, suggesting altered bacteriome functions. Patients had decreased levels of serotonin, GABA, dopamine, butyrate, and acetate in their stool samples compared to controls. Mycobiome analysis did not reveal significant differences in alpha diversity and fungal profile composition between patients with AN and healthy controls, nor any correlation of the fungal composition with the bacterial profile. Our results show the changed profile of the gut microbiome and its metabolites in patients with severe AN.
Permanent Link: http://hdl.handle.net/11104/0319592