Laser-induced breakdown spectroscopy as a straightforward bioimaging tool for plant biologists, the case study for assessment of photon-upconversion nanoparticles in Brassica oleracea L. plant

0541897 - ÚIACH 2022 RIV US eng J - Journal Article
Modlitbová, P. - Střítežská, Š. - Hlaváček, Antonín - Procházka, D. - Pořízka, P. - Kaiser, J.
Laser-induced breakdown spectroscopy as a straightforward bioimaging tool for plant biologists, the case study for assessment of photon-upconversion nanoparticles in Brassica oleracea L. plant.
Ecotoxicology and Enviromental Safety. Roč. 214, MAY (2021), č. článku 112113. ISSN 0147-6513. E-ISSN 1090-2414
R&D Projects: GA ČR(CZ) GJ18-03367Y
Institutional support: RVO:68081715
Keywords : rare-earth elements * spatial element distribution * translocation * bioaccumulation
OECD category: Analytical chemistry
Impact factor: 7.129, year: 2021
Method of publishing: Limited access

The main purpose of this work is to thoroughly describe the implementation protocol of laser-induced breakdown spectroscopy (LIBS) method in the plant analysis. Numerous feasibility studies and recent progress in instrumentation and trends in chemical analysis make LIBS an established method in plant bioimaging. In this work, we present an easy and straightforward phytotoxicity case study with a focus on LIBS method. We intend to demonstrate in detail how to manipulate with plants after exposures and how to prepare them for analyses. Moreover, we aim to achieve 2D maps of spatial element distribution with a good resolution without any loss of sensitivity. The benefits of rapid, low-cost bioimaging are highlighted.
Permanent Link: http://hdl.handle.net/11104/0319393