Linear dichroism imaging macros v2.0

0541871 - ÚOCHB 2022 RIV CZ eng L4 - Software
Lazar, Josef
Linear dichroism imaging macros v2.0.
Internal code: Linear dichroism imaging macros v2.0 ; 2020
Technical parameters: Software umožňující zpracování mikroskopických obrázků získaných technikami polarizační mikroskopie, včetně vizualizace a kvantitace lineárního dichroismu vykazovaného zobrazovanými vzorky, či určování molekulárních orientací na základě pozorovaného lineárního dichroismu.
Economic parameters: Software pro zpracování obrázků získaných technikami polarizační mikroskopie
R&D Projects: GA MŠMT(CZ) EF16_019/0000729
Institutional support: RVO:61388963
Keywords : polarization microscopy * linear dichroism
OECD category: Biophysics
https://github.com/jlazarlab/polarization_microscopy_macros/

Fluorescence-detected linear dichroism microscopy allows observing various molecular processes in living cells, as well as obtaining quantitative information on orientation of fluorescent molecules associated with cellular features. Such information can provide insights into protein structure, aid in development of genetically encoded probes, and allow determinations of lipid membrane properties. However, quantitating and interpreting linear dichroism in biological systems has been laborious and unreliable. Our open source ImageJ-based software tools now allow fast and easy linear dichroism visualization and quantitation, as well as extraction of quantitative information on molecular orientations, even in living systems.
Permanent Link: http://hdl.handle.net/11104/0319369


Research data: Zenodo