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Acetophenyl‐thienyl‐aniline‐Linked Nucleotide for Construction of Solvatochromic Fluorescence Light‐Up DNA Probes Sensing Protein‐DNA Interactions
- 1.0541740 - ÚOCHB 2022 RIV DE eng J - Journal Article
Güixens-Gallardo, Pedro - Hocek, Michal
Acetophenyl‐thienyl‐aniline‐Linked Nucleotide for Construction of Solvatochromic Fluorescence Light‐Up DNA Probes Sensing Protein‐DNA Interactions.
Chemistry - A European Journal. Roč. 27, č. 24 (2021), s. 7090-7093. ISSN 0947-6539. E-ISSN 1521-3765
R&D Projects: GA ČR(CZ) GA17-14791S; GA MŠMT(CZ) EF16_019/0000729
Grant - others:AV ČR(CZ) AP1501
Program: Akademická prémie - Praemium Academiae
Institutional support: RVO:61388963
Keywords : fluorescent labelling * DNA polymerases * nucleotides * protein-DNA interactions * solvatochromism
OECD category: Biochemistry and molecular biology
Impact factor: 5.020, year: 2021
Method of publishing: Limited access
https://doi.org/10.1002/chem.202100575
The synthesis of 2′‐deoxycytidine and its 5′‐O‐triphosphate bearing solvatochromic acetophenyl‐thienyl‐aniline fluorophore was developed using the Sonogashira cross‐coupling reaction as the key step. The triphosphate was used for polymerase synthesis of labelled DNA. The labelled nucleotide or DNA exerted weak red fluorescence when excited at 405 nm, but a significant colour change (to yellow or green) and light‐up (up to 20 times) was observed when the DNA probes interacted with proteins or lipids.
Permanent Link: http://hdl.handle.net/11104/0319263
Number of the records: 1