Growth Factors VEGF-A(165) and FGF-2 as Multifunctional Biomolecules Governing Cell Adhesion and Proliferation

0541641 - FGÚ 2022 RIV CH eng J - Journal Article
Sedlář, Antonín - Trávníčková, Martina - Matějka, Roman - Pražák, Šimon - Mészáros, Zuzana - Bojarová, Pavla - Bačáková, Lucie - Křen, Vladimír - Slámová, Kristýna
Growth Factors VEGF-A(165) and FGF-2 as Multifunctional Biomolecules Governing Cell Adhesion and Proliferation.
International Journal of Molecular Sciences. Roč. 22, č. 4 (2021), č. článku 1843. E-ISSN 1422-0067
R&D Projects: GA ČR(CZ) GA18-01163S; GA MZd(CZ) NV18-02-00422; GA MŠMT(CZ) LQ1604; GA MŠMT(CZ) ED1.1.00/02.0109; GA MŠMT(CZ) LTC18038
Institutional support: RVO:67985823 ; RVO:61388971
Keywords : heterologous expression * recombinant vascular endothelial growth factor (VEGF) * basic fibroblast growth factor (bFGF) * adult stem cells * endothelial cells * cell adhesion * cell proliferation * tissue engineering * regenerative medicine * vascular replacements
OECD category: Biomaterials (as related to medical implants, devices, sensors); Biochemistry and molecular biology (MBU-M)
Impact factor: 6.208, year: 2021
Method of publishing: Open access
https://www.mdpi.com/1422-0067/22/4/1843

Vascular endothelial growth factor-A(165) (VEGF-A(165)) and fibroblast growth factor-2 (FGF-2) are currently used for the functionalization of biomaterials designed for tissue engineering. We have developed a new simple method for heterologous expression and purification of VEGF-A(165) and FGF-2 in the yeast expression system of Pichia pastoris. The biological activity of the growth factors was assessed in cultures of human and porcine adipose tissue-derived stem cells (ADSCs) and human umbilical vein endothelial cells (HUVECs). When added into the culture medium, VEGF-A(165) stimulated proliferation only in HUVECs, while FGF-2 stimulated the proliferation of both cell types. A similar effect was achieved when the growth factors were pre-adsorbed to polystyrene wells. The effect of our recombinant growth factors was slightly lower than that of commercially available factors, which was attributed to the presence of some impurities. The stimulatory effect of the VEGF-A(165) on cell adhesion was rather weak, especially in ADSCs. FGF-2 was a potent stimulator of the adhesion of ADSCs but had no to negative effect on the adhesion of HUVECs. In sum, FGF-2 and VEGF-A(165) have diverse effects on the behavior of different cell types, which maybe utilized in tissue engineering.
Permanent Link: http://hdl.handle.net/11104/0319173