In Vitro Interactions of TiO2 Nanoparticles with Earthworm Coelomocytes: Immunotoxicity Assessment

0541602 - MBÚ 2022 RIV CH eng J - Journal Article
Navarro Pacheco, Natividad Isabel - Roubalová, Radka - Semerád, Jaroslav - Grasserová, Alena - Benada, Oldřich - Kofroňová, Olga - Cajthaml, Tomáš - Dvořák, Jiří - Bilej, Martin - Procházková, Petra
In Vitro Interactions of TiO2 Nanoparticles with Earthworm Coelomocytes: Immunotoxicity Assessment.
Nanomaterials. Roč. 11, č. 1 (2021), č. článku 250. E-ISSN 2079-4991
R&D Projects: GA MZe(CZ) QK1910095
EU Projects: European Commission(XE) 671881 - PANDORA
Research Infrastructure: Czech-BioImaging II - 90129
Institutional support: RVO:61388971
Keywords : earthworm * coelomocyte * TiO2 nanoparticles * reactive oxygen species * innate immunity * lipid peroxidation
OECD category: Environmental sciences (social aspects to be 5.7)
Impact factor: 5.719, year: 2021
Method of publishing: Open access
https://www.mdpi.com/2079-4991/11/1/250

Titanium dioxide nanoparticles (TiO2 NPs) are manufactured worldwide. Once they arrive in the soil environment, they can endanger living organisms. Hence, monitoring and assessing the effects of these nanoparticles is required. We focus on the Eisenia andrei earthworm immune cells exposed to sublethal concentrations of TiO2 NPs (1, 10, and 100 mu g/mL) for 2, 6, and 24 h. TiO2 NPs at all concentrations did not affect cell viability. Further, TiO2 NPs did not cause changes in reactive oxygen species (ROS) production, malondialdehyde (MDA) production, and phagocytic activity. Similarly, they did not elicit DNA damage. Overall, we did not detect any toxic effects of TiO2 NPs at the cellular level. At the gene expression level, slight changes were detected. Metallothionein, fetidin/lysenin, lumbricin and MEK kinase I were upregulated in coelomocytes after exposure to 10 mu g/mL TiO2 NPs for 6 h. Antioxidant enzyme expression was similar in exposed and control cells. TiO2 NPs were detected on coelomocyte membranes. However, our results do not show any strong effects of these nanoparticles on coelomocytes at both the cellular and molecular levels.
Permanent Link: http://hdl.handle.net/11104/0319135