Sustained photobiological hydrogen production by Chlorella vulgaris without nutrient starvation

0541579 - MBÚ 2022 RIV GB eng J - Journal Article
Touloupakis, E. - Faraloni, C. - Silva Benavides, A. M. - Masojídek, Jiří - Torzillo, G.
Sustained photobiological hydrogen production by Chlorella vulgaris without nutrient starvation.
International Journal of Hydrogen Energy. Roč. 46, č. 5 (2021), s. 3684-3694. ISSN 0360-3199. E-ISSN 1879-3487
Institutional support: RVO:61388971
Keywords : photosynthesis * fluorescence * photoproduction * inactivation * Photobiological hydrogen production * Chlorella * Light conversion efficiency * Photobioreactor * Dark hydrogen production
OECD category: Microbiology
Impact factor: 7.139, year: 2021
Method of publishing: Open access
https://www.sciencedirect.com/science/article/pii/S0360319920341550?via%3Dihub

This article describes the ability of the Chlorella vulgaris BEIJ strain G-120 to produce hydrogen (H-2) via both direct and indirect pathways without the use of nutrient starvation. Photobiological H-2 production reached a maximum rate of 12 mL H-2 L(-1 )h(-1), corresponding to a light conversion efficiency (light to H-2) of 7.7% (average 3.2%, over the 8-day period) of PAR, (photosynthetically active irradiance). Cells presented a maximum in vivo hydrogenase activity of 25.5 +/- 0.2 nmoles H-2 mu gChl(-1) h(-1) and the calculated in vitro hydrogenase activity was 830 +/- 61 nmoles H-2 mu gChl(-1) h(-1). The strain is able to grow either heterotrophically or photo autotrophically. The total output of 896 mL of H-2 was attained for illuminated culture and 405 mL for dark cultures. The average H-2 production rate was 4.98 mL L-1 h(-1) for the illuminated culture and 2.08 mL L-1 h(-)(1) for the one maintained in the dark. (C) 2020 Published by Elsevier Ltd on behalf of Hydrogen Energy Publications LLC.
Permanent Link: http://hdl.handle.net/11104/0326026