Hormonal responses associated with acclimation to freezing stress in Lolium perenne

0541511 - ÚEB 2022 RIV NL eng J - Journal Article
Přerostová, Sylva - Zupková, Barbara - Petřík, Ivan - Šimura, Jan - Našinec, I. - Kopecký, David - Knirsch, Vojtěch - Gaudinová, Alena - Novák, Ondřej - Vaňková, Radomíra
Hormonal responses associated with acclimation to freezing stress in Lolium perenne.
Environmental and Experimental Botany. Roč. 182, FEB (2021), č. článku 104295. ISSN 0098-8472. E-ISSN 1873-7307
R&D Projects: GA ČR(CZ) GA17-06613S; GA MŠMT(CZ) EF16_019/0000738
Institutional support: RVO:61389030
Keywords : Cold acclimation * Cold stress * Freezing stress * Lolium perenne * Phytohormone * RT-qPCR
OECD category: Plant sciences, botany
Impact factor: 6.028, year: 2021
Method of publishing: Open access
http://doi.org/10.1016/j.envexpbot.2020.104295

Complex phytohormonal analyses revealed hormone changes underlying the freezing response of perennial ryegrass (Lolium perenne L.). Comparisons among sensitive (code of accession 1/61), highly tolerant (5/101) and semi-tolerant (6/104, sensitive without acclimation, but tolerant after acclimation) genotypes allowed identification of changes associated with freezing tolerance. The main response to freezing stress (−7 °C for four days) was a significant elevation of abscisic acid (ABA) in all tissues of all genotypes, supported by elevation of jasmonic acid (JA) in leaves, and of salicylic acid (SA) in crowns and roots. Jasmonate-isoleucine increase was observed in roots of the tolerant genotype. The auxin (indole-3-acetic acid) levels showed relatively minor changes, while cytokinins exhibited up-regulation in crowns of sensitive genotypes. Tolerant genotypes exhibited decreased levels of highly active cytokinin trans-zeatin compensated by elevated low-active cis-zeatin, involved in stress responses. Cold acclimation (3 °C for seven days) applied prior frost treatment was associated with stimulation of plant defences, especially expression of ice recrystallisation inhibition protein LpIRI3. Acclimation diminished the impact of subsequent freezing treatment as indicated by lower up-regulation of stress hormones ABA, JA and SA in all tested tissues (with the exception of SA in crowns and roots of sensitive genotype) and up-regulation of active cytokinins in tolerant and semi-tolerant genotypes, especially in crowns and roots.
Permanent Link: http://hdl.handle.net/11104/0319074