Comparative Omics Analysis of Historic and Recent Isolates of Bordetella pertussis and Effects of Genome Rearrangements on Evolution

0541481 - MBÚ 2022 RIV US eng J - Journal Article
Dienstbier, Ana - Amman, F. - Petráčková, Denisa - Štipl, Daniel - Čapek, Jan - Zavadilová, J. - Fabiánová, K. - Držmíšek, Jakub - Kumar, Dilip - Wildung, M. - Pouchnik, D. - Večerek, Branislav
Comparative Omics Analysis of Historic and Recent Isolates of Bordetella pertussis and Effects of Genome Rearrangements on Evolution.
Emerging Infectious Diseases. Roč. 27, č. 1 (2021), s. 57-68. ISSN 1080-6040. E-ISSN 1080-6059
R&D Projects: GA MZd(CZ) NV16-30782A; GA ČR(CZ) GA19-12338S; GA MŠMT(CZ) ED1.1.00/02.0109
Grant - others:AV ČR(CZ) MSM200201702
Program: Program na podporu mezinárodní spolupráce začínajících výzkumných pracovníků
Institutional support: RVO:61388971
Keywords : secretion system * gene * vaccine * phosphoglucomutase * lipopolysaccharide * expression * virulence * alignment
OECD category: Microbiology
Impact factor: 16.126, year: 2021
Method of publishing: Open access
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7774529/

Despite high vaccination coverage, pertussis is increasing in many industrialized countries, including the Czech Republic. To better understand Bordetella pertussis resurgence, we analyzed historic strains and recent clinical isolates by using a comparative omics approach. Whole-genome sequencing showed that historic and recent isolates of B. pertussis have substantial variation in genome organization and form separate phylogenetic clusters. Subsequent RNA sequence analysis and liquid chromatography with mass tandem spectrometry analyses showed that these variations translated into discretely separated transcriptomic and proteomic profiles. When compared with historic strains, recent isolates showed increased expression of flagellar genes and genes involved in lipopolysaccharide biosynthesis and decreased expression of polysaccharide capsule genes. Compared with reference strain Tohama I, all strains had increased expression and production of the type III secretion system apparatus. We detected the potential link between observed effects and insertion sequence element-induced changes in gene context only for a few genes.
Permanent Link: http://hdl.handle.net/11104/0319030