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Omics Analysis of Blood-Responsive Regulon in Bordetella pertussis Identifies a Novel Essential T3SS Substrate
- 1.0541459 - MBÚ 2022 RIV CH eng J - Journal Article
Držmíšek, Jakub - Štipl, Daniel - Petráčková, Denisa - Večerek, Branislav - Dienstbier, Ana
Omics Analysis of Blood-Responsive Regulon in Bordetella pertussis Identifies a Novel Essential T3SS Substrate.
International Journal of Molecular Sciences. Roč. 22, č. 2 (2021), č. článku 736. E-ISSN 1422-0067
R&D Projects: GA ČR(CZ) GA19-12338S; GA MŠMT(CZ) EF17_050/0008376; GA MŠMT(CZ) ED1.1.00/02.0109
Institutional support: RVO:61388971
Keywords : Bordetella pertussis * blood exposure * omics analyses * gene expression * protein secretion
OECD category: Microbiology
Impact factor: 6.208, year: 2021
Method of publishing: Open access
https://www.mdpi.com/1422-0067/22/2/736
Bacterial pathogens sense specific cues associated with different host niches and integrate these signals to appropriately adjust the global gene expression. Bordetella pertussis is a Gram-negative, strictly human pathogen of the respiratory tract and the etiological agent of whooping cough (pertussis). Though B. pertussis does not cause invasive infections, previous results indicated that this reemerging pathogen responds to blood exposure. Here, omics RNA-seq and LC-MS/MS techniques were applied to determine the blood-responsive regulon of B. pertussis. These analyses revealed that direct contact with blood rewired global gene expression profiles in B. pertussis as the expression of almost 20% of all genes was significantly modulated. However, upon loss of contact with blood, the majority of blood-specific effects vanished, with the exception of several genes encoding the T3SS-secreted substrates. For the first time, the T3SS regulator BtrA was identified in culture supernatants of B. pertussis. Furthermore, proteomic analysis identified BP2259 protein as a novel secreted T3SS substrate, which is required for T3SS functionality. Collectively, presented data indicate that contact with blood represents an important cue for B. pertussis cells.
Permanent Link: http://hdl.handle.net/11104/0319012
Number of the records: 1