Influence of Lipid Membranes on α-Synuclein Aggregation

0541374 - ÚOCHB 2022 RIV US eng J - Journal Article
Kurochka, Andrii - Yushchenko, Dmytro A. - Bouř, Petr - Shvadchak, Volodymyr V.
Influence of Lipid Membranes on α-Synuclein Aggregation.
ACS Chemical Neuroscience. Roč. 12, č. 5 (2021), s. 825-830. ISSN 1948-7193. E-ISSN 1948-7193
R&D Projects: GA ČR(CZ) GJ18-06255Y; GA ČR(CZ) GA20-10144S; GA MŠMT(CZ) EF16_019/0000729
Institutional support: RVO:61388963
Keywords : amyloid * fibril * fibrillization * kinetics * lag time * liposomes
OECD category: Biophysics
Impact factor: 5.780, year: 2021
Method of publishing: Limited access
https://doi.org/10.1021/acschemneuro.0c00819

α-Synuclein is a neuronal protein involved in synaptic vesicle trafficking. During the course of Parkinson's disease, it aggregates, forming amyloid fibrils that accumulate in the midbrain. This pathological fibrillization process is strongly modulated by physiological interactions of α-synuclein with lipid membranes. However, the detailed mechanism of this effect remains unclear. In this work, we used environment-sensitive fluorescent dyes to study the influence of model lipid membranes on the kinetics of α-synuclein fibrillization. We observed that formation of the fibrils from α-synuclein monomers is strongly delayed even by small amounts of lipids. Furthermore, we found that membrane-bound α-synuclein monomers are not involved in fibril elongation. Hence, presence of lipids slows down fibril growth proportionally to the fraction of membrane-bound protein.
Permanent Link: http://hdl.handle.net/11104/0318940