Repetitive Detection of Aromatic Hydrocarbon Contaminants with Bioluminescent Bioreporters Attached on Tapered Optical Fiber Elements.

0541361 - ÚCHP 2022 RIV CH eng J - Journal Article
Zajíc, J. - Ripp, S. - Trögl, J. - Kuncová, Gabriela - Pospíšilová, M.
Repetitive Detection of Aromatic Hydrocarbon Contaminants with Bioluminescent Bioreporters Attached on Tapered Optical Fiber Elements.
Sensors. Roč. 20, č. 11 (2020), č. článku 3237. E-ISSN 1424-8220
Institutional support: RVO:67985858
Keywords : whole-cell biosensor * bioluminescent bioreporter * optical fiber biosensor
OECD category: Bioprocessing technologies (industrial processes relying on biological agents to drive the process) biocatalysis, fermentation
Impact factor: 3.576, year: 2020
Method of publishing: Open access
https://www.mdpi.com/1424-8220/20/11/3237

In this study, we show the repetitive detection of toluene on a tapered optical fiber element (OFE) with an attached layer of Pseudomonas putida TVA8 bioluminescent bioreporters. The bioluminescent cell layer was attached on polished quartz modified with (3-aminopropyl)triethoxysilane (APTES). The repeatability of the preparation of the optical probe and its use was demonstrated with five differently shaped OFEs. The intensity of measured bioluminescence was minimally influenced by the OFE shape, possessing transmittances between 1.41% and 5.00%. OFE probes layered with P. putida TVA8 were used to monitor liquid toluene over a two-week period. It was demonstrated that OFE probes layered with positively induced P. putida TVA8 bioreporters were reliable detectors of toluene. A toluene concentration of 26.5 mg/L was detected after <30 min after immersion of the probe in the toluene solution. Additional experiments also immobilized constitutively bioluminescent cells of E. coli 652T7, on OFEs with polyethyleneimine (PEI). These OFEs were repetitively induced with Lauria-Bertani (LB) nutrient medium. Bioluminescence appeared 15 minutes after immersion of the OFE in LB. A change in pH from 7 to 6 resulted in a decrease in bioluminescence that was not restored following additional nutrient inductions at pH 7. The E. coli 652T7 OFE probe was therefore sensitive to negative influences but could not be repetitively used.
Permanent Link: http://hdl.handle.net/11104/0318921