Kinetic Model of the Action of 17 alpha-Ethynylestradiol on the Capacitation of Mouse Sperm, Monitored by HPLC-MS/MS

0541327 - BTÚ 2021 RIV CH eng J - Journal Article
Bosakova, T. - Tockstein, A. - Šebková, Nataša - Čabala, R. - Komrsková, Kateřina
Kinetic Model of the Action of 17 alpha-Ethynylestradiol on the Capacitation of Mouse Sperm, Monitored by HPLC-MS/MS.
Catalysts. Roč. 10, č. 1 (2020), č. článku 124. E-ISSN 2073-4344
R&D Projects: GA ČR(CZ) GA18-11275S; GA MŠMT(CZ) ED1.1.00/02.0109
Institutional support: RVO:86652036
Keywords : 17 alpha-ethynylestradiol * EE2 * sperm capacitation * kinetics
OECD category: Physical chemistry
Impact factor: 4.146, year: 2020
Method of publishing: Open access
https://www.mdpi.com/2073-4344/10/1/124

17 alpha-Ethynylestradiol (EE2), a synthetic estrogen used in contraceptive pills, is resistant to hepatic degradation and is excreted in the urine. It is chemically stable and has a negative impact on the endocrine system. The aim of this work was to mathematically describe the possible interaction of EE2 (200, 20, and 2 mu g/L) with sperm estrogen receptors during sperm maturation, which is called capacitation. The concentrations of the unbound EE2 remaining in capacitating medium during 180 min of sperm capacitation were determined at 30 min intervals by high performance liquid chromatography with tandem mass spectrometric detection (HPLC-MS/MS) and the data obtained (relative concentrations B-t) were subjected to kinetic analysis. The suggested kinetic schema was described by the system of differential equations with the optimization of rate constants used to calculate the theoretical B-t values. Optimal parameters (overall rate constants K-1-K-5 and molar ratio n) were determined by searching the minimum of absolute values of the difference between theoretical and experimental B-t values. These values were used for the design of the theoretical B(t) curves which fit to experimental points. The proposed kinetic model assumes the formation of an unstable adduct between EE2 and the receptor in cytoplasm, which acts as an autocatalytic agent and gradually decomposes.
Permanent Link: http://hdl.handle.net/11104/0318898