Self-assembly of bovine serum albumin (BSA)-dextran bio-nanoconjugate: structural, antioxidant and in vitro wound healing studies

0541280 - ÚŽFG 2022 RIV GB eng J - Journal Article
Rohiwal, Sonali Suresh - Ellederová, Zdeňka - Tiwari, A. P. - Alqarni, M. - Elazab, S. T. - El-Saber Batiha, G. - Pawar, S. H. - Thorat, N. D.
Self-assembly of bovine serum albumin (BSA)-dextran bio-nanoconjugate: structural, antioxidant and in vitro wound healing studies.
RSC Advances. Roč. 11, č. 8 (2021), s. 4308-4317. E-ISSN 2046-2069
R&D Projects: GA MŠMT(CZ) LO1609
Institutional support: RVO:67985904
Keywords : bovine serum albumin * dextran * wound healing
OECD category: Biochemistry and molecular biology
Impact factor: 4.036, year: 2021
Method of publishing: Open access
https://pubs.rsc.org/en/content/articlelanding/ra/2021/d0ra09301g#!divAbstract

Glycation of proteins is often considered as a method to improve their functional properties for promising applications in wound healing. Furthermore, a marked increase in percentage of radical scavenging activity of the conjugates makes it an effective antioxidant synthetic strategy. A simple conjugation process was employed to develop bovine serum albumin-dextran conjugates (BSA-dextran) using Maillard reaction. Higher electrophoretic mobility and surface charge in the prepared conjugates was observed in native PAGE electrophoresis and zeta potential. Moreover, the fluorescence, FTIR and Raman analysis of the BSA-dextran conjugates shows significant shift in the fluorescence and wavelength as a consequence of conjugate formation. In vitro wound healing assay showed increased cell proliferation and migration effect. These finding suggests that BSA-dextran conjugate could open up a new practical way for exploration in the area of wound healing.
Permanent Link: http://hdl.handle.net/11104/0318864