Iodination of CART(61-102) peptide: Preserved binding and anorexigenic activity in mice

0541244 - ÚOCHB 2022 RIV GB eng J - Journal Article
Pražienková, Veronika - Marek, Aleš - Maletínská, Lenka
Iodination of CART(61-102) peptide: Preserved binding and anorexigenic activity in mice.
Journal of Labelled Compounds and Radiopharmaceuticals. Roč. 64, č. 2 (2021), s. 61-63. ISSN 0362-4803. E-ISSN 1099-1344
Institutional support: RVO:61388963
Keywords : CART peptide * PC12 cells * competitive binding experiments * food intake
OECD category: Biochemistry and molecular biology
Impact factor: 1.949, year: 2021
Method of publishing: Limited access
https://doi.org/10.1002/jlcr.3871

CART (cocaine- and amphetamine-regulated transcript) peptides are involved in food intake regulation, stress, and other physiological functions. Although CART peptides have been known for over 25 years, their receptor(s) have not yet been characterized. In this short review, we will summarize our previous studies, where we reported specific binding of 125 I-CART(61-102) to PC12 rat pheochromocytoma cells. Competitive binding experiments performed with mono- and di-iodinated peptides and their isoforms with oxidized Met67 resulted in nanomolar binding affinity. Moreover, in our previous study, CART(61-102), as well as di-iodinated CART(61-102), have shown a strong anorexigenic effect in fasted lean mice after intracerebroventricular administration. In conclusion, from our previous studies, iodination of CART(61-102) resulted in mono- and di-iodinated analogs with or without oxidized Met67 . All analogs revealed a high affinity to binding sites at PC12 cells and preserved biological activity.
Permanent Link: http://hdl.handle.net/11104/0318832