Zwitterionic polyaspartamides based on L-lysine side-chain moieties: synthesis, nonfouling properties and direct/indirect nanogel preparation

0540931 - ÚMCH 2022 RIV GB eng J - Journal Article
Hladysh, Sviatoslav - Oleshchuk, Diana - Dvořáková, Jana - Golunova, Anna - Šálek, Petr - Pánek, Jiří - Janoušková, Olga - Kaňková, Dana - Pavlova, Ewa - Proks, Vladimír
Zwitterionic polyaspartamides based on L-lysine side-chain moieties: synthesis, nonfouling properties and direct/indirect nanogel preparation.
European Polymer Journal. Roč. 148, 5 April (2021), č. článku 110347. ISSN 0014-3057. E-ISSN 1873-1945
R&D Projects: GA ČR(CZ) GA18-03224S
Institutional support: RVO:61389013
Keywords : polyaspartamide * zwitterionic polypeptide * polysuccinimide
OECD category: Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials
Impact factor: 5.546, year: 2021
Method of publishing: Limited access
https://www.sciencedirect.com/science/article/pii/S0014305721000811?via%3Dihub

The ability of polysuccinimide (PSI) to open the succinimide ring by nucleophilic reagents leads to the formation of various polyaspartamides under mild conditions. In the current work, amino acids, such as Nα-Boc-L-lysine, leading to the formation of exclusively α-substituted L-lysine polypeptides and L-lysine methyl ester were used as the ring-opening reagents for PSI. The formed materials possess both positively and negatively charged groups on the same repeat unit simultaneously serving as zwitterionic moieties. The chemical structure and component composition were confirmed by 1H NMR and UV/vis spectroscopies. Zwitterionic properties of the synthesized polymers were investigated by zeta potential measurements. The obtained zwitterionic polyaspartamides exhibited anti-protein adsorption properties and the ability to create hydrophilic surfaces. The zwitterionic polyaspartamide nanogels obtained by horseradish peroxidase-mediated crosslinking in inverse miniemulsions and applying different preparation strategies were analysed by dynamic light scattering (DLS) measurements and transmission electron microscopy (TEM) analysis. The results indicated the good morphology and architecture of the nanogels. These peptide-based zwitterionic nanogels, due to their good biocompatibility and anti-protein adsorption ability, are promising materials for biomedical applications.
Permanent Link: http://hdl.handle.net/11104/0319036